지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수0
2019
2018
I. INTRODUCTION 11. Prostate 11.1 Roles and structure of prostate 11.2 Development of prostate 41.3 Role of androgen receptor and testosterone in prostate 52. What is NKX3.1? 72.1 NKX3.1 transcription factor 72.2 Structure properties of the NKX3.1 gene and protein 83. Production and phenotypes of NKX3.1 KO mice 153.1. Production strategy for NKX3.1 KO mice 153.2. Phenotypic features of NKX3.1 KO mice 17II. MATERIALS AND METHODS 241. Phenotypical characterization of C57BL/6N-NKX3.1em1Hlee KO mice 241.1 Production and identification 241.2 Animals care and use 261.3 Analysis for litter size, sex composition and genotype 261.4 Measurement of body and organs weight 281.5 Measurement of food intake and water consumption 281.6 Analysis for survival rate and clinical sign 281.7 Whole blood and serum analysis 291.8 Measurement of body temperature and body length 301.9 Histological analysis 301.10 Immunohistochemical analysis (IHC) 311.11 Immunofluorescence analysis (IF) 311.12 Western blot 321.13 Enzyme-linked immunosorbent assay (ELISA) 331.14 Statistical analysis 332. Incidence of testosterone-induced prostate cancer in the CRISPR/Cas9-mediated C57BL/6-NKX3.1em1Hlee KO mice 342.1 Experimental design 342.2 Western blot 35III. RESULTS 361. Phenotypical characterization of C57BL/6N-NKX3.1em1Hlee KO mice 361.1 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on the genotype and sex ratio 361.2 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on the survival rate until 16-week-old 361.3 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on body temperature, body length and organs weight in 16-week-old 391.4 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on weight gain and feeding behavior until 16-week-old 391.5 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on blood and serum components in 16-week-old 451.6 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on weights in prostate 451.7 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on confirmation ofhistological difference 501.8 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on EGFR downstream signaling pathway 591.9 Effects of CRISPR/Cas9-mediated NKX3.1 deletion on apoptotic response and tumor related genes 592. Incidence of testosterone-induced prostate cancer in the CRISPR/Cas9-mediated C57BL/6-NKX3.1em1Hlee KO mice 732.1 Effects of testosterone injection on testosterone level in serum 732.2 Effects of testosterone injection on alteration in the body weight and feeding behavior 732.3 Effects of testosterone injection on conformation and weights of prostate 782.4 Effects of testosterone injection on histopathology of prostate each lobe 782.5 Effects of testosterone injection on expression of proteins in prostate 81IV. DISCUSSION 881. Phenotypical characterization of C57BL/6N-NKX3.1em1Hlee KO mice 882. Incidence of testosterone-induced prostate cancer in the CRISPR/Cas9-mediated C57BL/6N-NKX3.1em1Hlee KO mice 91V. REFERENCES 98SUMMARY (In Korean) 110Curriculum Vitae 113
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