지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 鄭晶旭
- 발행연도
- 2019
- 저작권
- 안동대학교 논문은 저작권에 의해 보호받습니다.
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In this study, to reduce the production cost of poly(3-hydroxyalkanoates) (PHA), optimal growth and PHA biosynthesis conditions for the production of Pseudomonas sp. EML2 and Pseudomonas sp. EML8 were established using waste frying oil (WFO) as the cheap carbon source. The fatty acid composition of WFO and fresh frying oil (FFO) were analyzed by gas chromatography (GC). Unsaturated and saturated fatty acid contents of FFO were found to be 82.6% and 14.9%, respectively. However, these contents were changed in WFO. The compositional change of unsaturated fatty acid in WFO was determined to be due to the change of chemical and physical properties resulting from heating, oxidation reaction and hydrolysis. The maximum dry cell weight (DCW) and PHB yield (g/L) of the isolated strain Pseudomonas sp. EML2 were confirmed under WFO 30 g/L, NH4Cl 0.5g/L, pH 7 and 20°C culture conditions. Based on this, the growth and PHB yield of Pseudomonas sp. EML2 were confirmed by 3 L jar fermentation. After the cells were cultured in WFO 30 g/L for 96 hours, DCW, PHB content and PHB yield of Pseudomonas sp. EML2 were 3.6 g/L, 73 wt.% and 2.6 g/L, respectively. Similarly, results were obtained using 30 g/L of fresh frying oil (FFO) as a control carbon source. In this case, DCW, PHB content and PHB yield were 3.4 g/L, 70 wt.%, and 2.4 g/L, respectively. GC and GC mass spectrometry analysis showed that mcl-PHA obtained by Pseudomoas sp. EML8 at WFO consists of 6.06 mol% 3-hydrxoyhexanoate, 32.50 mol% 3-hydroxyoctanoate, 30.89 mol% 3-hydroxydecanoate, 16.75 mol% 3-hydorxy-3,6-dodecadienoateand and 13.80 mol% 3-hydoryxdodecanoate monomers. The mcl-PHA monomer with 2 double bonds, 3-hydorxy-3, 6-dodecadienoateand, is depicted a new monomer that has not been reported. The maximum DCW and mcl-PHA yield (g/L) of the isolated strain Pseudomonas sp. EML2 were confirmed under culturing conditions of WFO 20 g/L, (NH4)2SO4 0.5g/L, pH 7 and 25°C. When was cultured with 3 L jar fermenter based on the above conditions, DCW, mcl-PHA content and mcl-PHA yield of Pseudomonas sp. EML8 were 3.0 g/L, 62 wt% and 1.9 g/L. Similarly, results were obtained using 20 g/L of FFO as a control carbon source. In this case, DCW, mcl-PHA content and mcl-PHA yield were 2.7 g/L, 62 wt%, and 1.6 g/L, respectively. In conclusion, the presence of Pseudomonas sp. EML2, Pseudomonas sp. EML8 and WFO were confirmed as a new candidate and substrates for industrial production of PHA.
목차
List of Abbreviations ------------------------------------------------ ⅲList of Tables ---------------------------------------------------------- ⅳList of Figures --------------------------------------------------------- ⅴⅠ.서론 ------------------------------------------------------------------ 1Ⅱ.재료 및 방법 --------------------------------------------------------- 71. 탄소원 --------------------------------------------------------------- 72. 균주 ------------------------------------------------------------------ 73. 16S ribosomal DNA (16S rDNA) 염기서열 결정 -------------- 74. Flask를 이용한 최적 배양조건 확립 ------------------------------- 94-1) 배양시간 -------------------------------------------------------- 94-2) 배양온도 -------------------------------------------------------- 94-3) pH --------------------------------------------------------------- 94-4) 질소원 ----------------------------------------------------------- 104-5) 탄소원 농도 ----------------------------------------------------- 105. Fermentor를 이용한 PHA의 생합성 ------------------------------- 105-1) Fermentation 조건 -------------------------------------------- 105-2) 탄소원 잔량 측정 ------------------------------------------------ 115-3) 질소원 잔량 측정 ------------------------------------------------ 116. WFO와 FFO의 지방산 분석 ----------------------------------------- 117. PHA의 분석 ----------------------------------------------------------- 147-1) GC와 GC/Mass Spectrometry (GC/MS)에 의한 분석 ----- 147-2) PHA의 물성 분석 ------------------------------------------------ 16Ⅲ.결과 및 고찰 ----------------------------------------------------------- 171. Pseudomonas sp. EML2의 PHB 생합성 -------------------------- 171-1) WFO와 FFO의 지방산 조성 ------------------------------------ 171-2) 균주 분리 및 동정 ------------------------------------------------ 191-3) Flask를 이용한 최적 배양조건 확립 ---------------------------- 191-4) Fermentor를 이용한 PHB 생합성 ----------------------------- 311-5) PHB의 물성 ------------------------------------------------------ 332. Pseudomonas sp. EML8의 mcl-PHA 생합성 ------------------- 382-1) 균주 분리 및 동정 ----------------------------------------------- 382-2) Flask를 이용한 최적 배양조건 확립 --------------------------- 412-3) Fermentor를 이용한 mcl-PHA 생합성 ----------------------- 462-4) mcl-PHA의 물성 ------------------------------------------------ 51Ⅳ.요약 -------------------------------------------------------------------- 57Ⅴ.참고문헌 --------------------------------------------------------------- 60Ⅵ. Abstract -------------------------------------------------------------- 66
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