Ginseng is a perennial medicinal plant and the most widely known medicinal plant in the world. It is known that ginsenoside, a kind of saponin contained in ginseng, is effective for various adult diseases and diabetes, solid pressure, malignant tumor, sexual function inhibition. However, recently, due to the changes in consumption patterns of ginseng, the ginseng market is gradually being reduced. As a result, the market for sprout ginsengs, which is a new form of consumption of ginseng, is gradually getting larger in order to stimulate consumption of ginseng. The sprout ginseng is a new consumption form that uses ginseng as a fresh vegetable, and it has the advantage that it can ingest the ginsenosides of the shoot of ginseng which has been abandoned. This study was carried out to investigate the effect of medium combinations and ozone gas treatment on the growth of sprout ginseng and changes in ginsenoside content, as the purpose of cultivating high-quality sprout ginseng using plant factory. As the first experiment, an artificial low temperature storage was applied to reduce the dormancy period of 1-year-old ginseng. The ginsengs were stored for 45 days and 90 days at low temperature storage temperature of ?2, 0 and 3℃. The highest germination ability was obtained when stored for 90 days at low temperature for 3℃. In the second experiment, the growth and morphological characteristics of the sprout ginseng were investigated according to the combinations of media at 4 weeks, 5 weeks and 6 weeks after transplanting. In addition, the total ginsenoside of the shoot and root of the sprout ginseng was measured, respectively, at 4 weeks after transplanting. The combinations of media used in second experiment were five, different ratio of commercial soil and perlite, 100:0, 75:25, 50:50, 25:75, and 0:100. As a result, the highest growth was found at 75:25 and the morphological chracteristics was showed as the highest quality at 4 weeks after transplanting. The total ginsenoside concentrations of the shoot and root of the sprout ginseng was also highest at 75:25. The third experiment was conduncted to increase the total ginsenoside of sprout ginseng using ozone gas treatment. Before harvesting, the sprout ginseng was treated with 1 ppm ozone gas concentration during 0(con), 1.5, 3, 6, 12, and 24 hours. Experimental results showed the highest ginsenoside concentrations at 1 ppm 3 hours. Based on the results of the third experiment, the last experiments were conducted. The treatments were consisted of 0.5 ppm for 6 hours, 1 ppm for 3 hours, 2 ppm for 1.5 hours, and 3 ppm for 1 hour. In addition, in order to observe the change of total ginsenoside concentrations by different ozone gas concentration, ozone gas treatment was performed for 3 hours at 0.5 ppm, 1 ppm, 2 ppm, 3 ppm. As a result of the last experiment, total ginsenoside concentrations were the highest for 6 hour at 0.5 ppm and 3 hour at 0.5 ppm. The results of these study show that short term stress treatment before crop harvesting can increase the medicinal compounds without affecting biomass. In the future, in order to increase the ginsenoside content of ginseng, it is necessary to carry out an experiment on low concentration ozone gas treatment at the level of 0.5 ppm.