지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 발행연도
- 2017
- 저작권
- 서울대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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The basic leucine zipper (bZIP) TF family is one of the largest plant-specific TF families and particularly, group-A Arabiodpsis bZIP TFs are known to be involved in abiotic stress responses. In this study, we isolated and functionally characterized OsbZIP66 which belongs to the group-A AtbZIP TFs. To investigate the response of OsbZIP66 to abiotic stresses, quantitative RT-PCR was performed and resulted that the transcriptional expression of OsbZIP66 is induced by drought stress and ABA. Examination of putative OsbZIP66 promoter revealed that there are several ABA-responsive elements (ABREs), suggesting that OsbZIP66 regulates drought stress in an ABA-dependent manner. Further phenotypic and chlorophyll fluorescence analysis proved that overexpression of OsbZIP66 in both whole plant body and specifically in root enhanced drought tolerance in rice. These results indicate that the drought tolerance of rice has positive correlation with the expression level of OsbZIP66 and the overexpression of OsbZIP66 in roots is important for drought tolerance. Taken together, OsbZIP66 has a high potential to improve drought tolerance in rice.
목차
INTRODUCTION 1MATERIALS AND METHODS 41. Phylogenetic analysis and multiple sequence alignment 42. Plasmid construction and rice transformation 43. ABA hormone treatment 54. RNA isolation and cDNA syntehsis for qRT-PCR 65. Subsellular localization in rice protoplasts 66. Drought stress treatment and phenotype analysis 87. PAM test for drought tolerance validation 98. Molecular characterization of T-DNA insertional mutant 99. Agrnomic trait analysis with the rice plants of reproductive stage 10RESULTS 121. Isolation and identification of the OsbZIP66 TF 122. Responsiveness of OsbZIP66 to various abiotic stresses 123. Expression of OsbZIP66 is induced by ABA 134. Temporal expression patterns and nuclear localization of OsbZIP66 145. OsbZIP66 expression level in transgenic rice plants 146. Overexpression rice plants of OsbZIP66 improve drought tolerance 157. Molecular characterization of a T-DNA insertional mutant 168. Possible roles of OsbZIP66 in rice development 17DISCUSSION 43REFERENCES 46ABSTRACT IN KOREAN 51APENDIX 53
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