The ethanol extracts of Erigeron annuus (L.) Pers. were investigated for the activities of whitening effects to apply as a functional ingredient for cosmetic products. The electron donating ability of 70% ethanol extract from Erigeron annuus (L.) Pers. shown 67.83% at 1,000 μg/ml concentration. The ABTS+ radical scavenging ability of shown 87% at 500 μg/ml concentration. The tyrosinase inhibitory effect which is related to skin-whitening, was 69% at the concentration of 1,000 μg/ml. The elastase inhibitory effect which is related to skin-wrinkle, was 69% at 1,000 μg/ml concentration. The astringent effect was 80% at 1,000 μg/ml concentration. For a cell viability test, measured on melanoma cell by ethanol extract of Erigeron annuus (L.) Pers., results showed 96% with cell viability at 100 μg/ml concentration. According to the results of western blot of ethanol extract from Erigeron annuus (L.) Pers. the expression of the MITF, tyrosinase, TRP-1, TRP-2 protein was decreased by 60.22%, 47.83%, 54.79%, 67.88% at 100 μg/ml concentration. In the p-ERK and p-CREB, protein expression was decreased with increasing concentrations of the Erigeron annuus (L.) Pers. extract. RT-PCR of ethanol extract from Erigeron annuus (L.) Pers. showed that the expression of MITF, tyrosinase, TRP-1, TRP-2 mRNA was decreased by 86.51%, 85.22%, 74.26%, 66.66% at 100 μg/ml concentration. Erigeron annuus (L.) Pers. for the multiple emulsion was prepared containing. The results of stability test showed that the W/O/W multiple emulsion containing Erigeron annuus (L.) Pers. extract were stable at accelerated temperature conditions. In addition, pH, viscosity and particle stability of multiple emulsion did not change for 30 days. The findings suggest that 70% ethanol extract from Erigeron annuus (L.) Pers. has potential as a cosmeceutical ingredient with whitning effect.