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논문 기본 정보

자료유형
학위논문
저자정보

정안나 (전남대학교, 전남대학교 대학원)

지도교수
오세종
발행연도
2017
저작권
전남대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (2)

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Gamma-aminobutyric acid (GABA) is a non-proteinogenic amino acid synthesized by microorganisms through decarboxylation of glutamate by glutamate decarboxylase (GAD). Probiotics are defined as viable microorganisms that have a the health benefit on humans by maintaining or improving their intestinal microbial balance. The purpose of this study was to investigate the production of GABA from traditional fermented foods (kimchi, etc.) by lactic acid bacteria. A GABA-producing strain was isolated from home-made kimchi and was identified as Lactobacillus brevis by 16S rDNA gene sequencing. It is well established that gadB (encoding glutamate decarboxylase) and, gadC (encoding a glutamate/GABA antiporter) are important for the production of GABA. The gadB and gadC levels were determined by qRT-PCR. The expression level of the gadB gene was highest at 18 h, whereas the expression level of gadC increased depending on the incubation time. This result suggests that Lactobacillus brevis Bmb5 has the potential probiotics to be utilized as a strain for GABA production.

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CONTENTS
List of Figures Ⅲ
List of tables Ⅴ
Abstract Ⅵ
1. Introduction 1
2. Materials and Methods 3
2.1 Measuring acid tolerance 3
2.2 Measuring MSG tolerance 3
2.3 GABA measurement with GABAse 3
2.4 GABA assay by HPLC 4
2.5 Identification of GABA-Producing LAB 4
2.6 RNA isolation and RT-PCR experiment 5
2.7 Growth of the L. brevis Bmb5 6
2.8 Statistical analysis 6
3. Results and Discussion 7
3.1. Selection of acid and bile tolerance strains 7
3.2. Screening of LAB with MSG tolerance 9
3.3. Evaluation of GABA content by GABAse assay 11
3.4. Analysis of GABA contents by HPLC 13
3.5. Growth characteristics of L. brevis Bmb5 17
3.6. Expression of L. brevis Bmb5 gadB and gadC genes 19
4. References 22
5. Abstract(Korean) 27
6. Acknowledgements 29

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