In this study, the effect that reaches the inflammation of ethanol extracts from Caryopteris incana and fermented Caryopteris incana on induced to LPS with Raw 264.7 Cell was verified. To select optimum extraction condition, Caryopteris incana was extracted by several sorts of solvents and concentrations of solvents. The optimum conditions for extracting phenolic compounds were to extract using 80% ethanol. Cell cytotoxicity was identified by treating purified Caryopteris incana and fermented Caryopteris incana in Raw 264.7 cell at different concentrations (5, 10, 15, 20, 30, 40, 50 ㎍/mL). As a result, 5, 10, 15 ㎍/mL of Caryopteris incana and 10, 20, 30, 40 ㎍/mL of fermented Caryopteris incana was shown none effect on cell viability. Therefore, iNOS and COX-2 protein expression, NO and PGE2 production, and cytokine were conducted with group under concentration in which it doesn''t have an effect on the cell viability. Determining iNOS protein expression, both Caryopteris incana and fermented Caryopteris incana was showed 50% 15 ㎍/mL, 40 ㎍/mL concentration respectively. Determining COX-2 protein expression, Caryopteris incana was showed 50% 15 ㎍/mL concentration. And fermented Caryopteris incana was showed 17% 40 ㎍/mL concentration. To investigate NO production inhibiton, Caryopteris incana was showed 50% 15 ㎍/mL concentration. And fermented Caryopteris incana was showed 17% 40 ㎍/mL concentration even inhibited production level which is similar to the normal group. To identify PGE2 production inhibitory effect, Caryopteris incana and fermented Caryopteris incana was treated at respective concentration of 15 ㎍/mL, 30 ㎍/mL exhibited inhibition of PGE2 production aspects 30%. Protein expression of NO bio-synthesizing enzyme iNOS and PGE2 bio-synthesizing enzyme COX-2 was similar to the inhibitory effect. To determine of Pro-inflammatory cytokine down regulation, TNF-α, IL-1β, IL-6 was measured production expression. In the case of TNF-α, Caryopteris incana was showed 60% 15 ㎍/mL concentration. And fermented Caryopteris incana was showed 35% 40 ㎍/mL concentration. In the case of IL-6, both Caryopteris incana and fermented Caryopteris incana was showed 35% 15 ㎍/mL,40 ㎍/mL concentration respectively. In the case of IL-1β, Caryopteris incana was showed 48% 15 ㎍/mL concentration. And fermented Caryopteris incana was showed 30% 40 ㎍/mL concentration.
These results show that fermented Caryopteris incana was the more reduced cytotoxic than Caryopteris incana and both Caryopteris incana and fermented Caryopteris incana inhibits cell inflammation by decreasing the expression and secretion of inflammation inducing substances in Raw 264.7 cell induced by LPS. Therefore, it was found that both Caryopteris incana and fermented Caryopteris incana can be used as an excellent material for the development of new anti-inflammatory drugs.