개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 허강무
- 발행연도
- 2016
- 저작권
- 충남대학교 논문은 저작권에 의해 보호받습니다.
이용수0
이 논문의 연구 히스토리 (3)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Over the past years, thermo-sensitive polymers that undergo thermo-responsive sol-gel transition in aqueous media have been studied for various biomedical applications, especially drug delivery systems, cell carriers and Scaffolds for tissue regeneration. Glycol chitosan (GC) is a derivative of chitosan that is soluble at neutral pH and possesses potentially useful biological properties. In this study, we synthesized hexanoyl glycol chitosan (HGC) as a thermo-sensitive polymer by N-hexanoylation of glycol chitosan using hexanoic anhydride. The aqueous solution of HGC (36% of degree of substitution, 4 wt%) showed a sol-gel transition around 32°C. However, the major limitations of HGC for biomedical applications are low gel stability and weak mechanical properties under physiological conditions. Therefore, we synthesized a photo-crosslinkable thermo-sensitive polymer, methacrylated hexanoyl glycol chitosans (M-HGCs), by N-methacrylation of hexanoyl glycol chitosan. The degree of methacrylation was controlled to have a range of 3.6%~19.5% by varying the feed molar ratio of reactants. The sol-gel transition temperature of M-HGCs decreased slightly with the introduction of hydrophobic methacryl groups. M-HGCs could form thermogels that can be chemically crosslinked by UV exposure to have enhanced mechanical properties. From in vitro cytotoxicity study no significant toxicity was observed ad more than 90% cells were viable. Also, Chondrocytes encapsulation into M-HGC hydrogel and remained viable for up to one week. These crosslinkable thermogel systems are expected to be a promising biomaterial for drug delivery system and various biomedical applications
목차
1. Introduction 11.1 Hydrogel 11.2 Thermo-sensitive polymer 21.2.1 Definition of thermo-sensitive polymers 21.2.2 Synthetic polymer-based thermo-sensitive polymers 41.2.3 Natural polymer-based thermo-sensitive polymers 71.3 Chitosan based thermo-sensitive polymers 91.4 Photo-crosslinkable thermo-sensitive polymer 111.5 Research rationale 122. Experiment 132.1 Materials 132.2 Synthesis of photo-crosslinkable thermo-sensitive polymer 132.2.1 Synthesis of hexanoyl glycol chitosan 132.2.2 Synthesis of methacrylated hexanoyl glycol chitosan 142.3 Characterization of HGC and M-HGCs 142.4 Photo-crosslinking of M-HGC hydrogels 142.5 Thermo-sensitive sol-gel transition temperature 152.6 Swelling profile 152.7 Hydrogel morphology 162.8 Enzymatic degradation 162.9 Rheological studies 172.10 Isolation of chondrocytes 172.11 In vitro cytotoxicity assays 183. Results and Discussion 193.1 Synthesis and characterization of HGC and M-HGC 193.2 Thermo-sensitive sol-gel transition properties 233.3 Photo-crosslinking of M-HGCs 273.4 Mechanical properites of HGC and M-HGC hydrogels 303.5 Swelling and morphology studies of M-HGC hydrogels 323.6 Enzymatic degradation 363.7 Cell encapsulation 384. Conclusion 40References 41
최근 본 자료
댓글(0)
0