지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 천철홍
- 발행연도
- 2016
- 저작권
- 고려대학교 논문은 저작권에 의해 보호받습니다.
이용수0
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
5-Androsten-3β-,17β-diol-7-one diacetate is an anabolic steroid derived from dehydroepiandrosterone. Anabolic steroids, intraglandular precursors of hormones, usually exhibit minimal hormonal effects by themselves. They are known to increase muscle growth and strength, and bodybuilders often use them. However, they can cause gynecomastia, virilism, and skin diseases as side effects. Even these side effects steroids are being developed. In this study, new metabolites and metabolic processes are investigated for determining safety of drugs. 5-Androsten-3β-,17β-diol-7-one diacetate as unknown metabolites was orally administrated to six healthy volunteers. And then samples were prepared from urine by way of PAD-1 adsorption, enzymatic hydrolysis with β-glucuronidase, and liquid/liquid extraction. As a result of optimizing the pretreatment, it was extracted with diethyl ether at pH 6.8 and derivatized with MSTFA/NH4I/DTE (500 : 2 : 1 (w/w/w)) at 60 ℃ for 15 min. The extracted 5-androsten-3β-,17β-diol-7-one diacetate and its metabolites were compared to blank urine, spiked urine, and dosed urine samples by LC/MS and GC/MS. 5-Androsten-3β-,17β-diol-7-one diacetate was not detected in dosed urine, but M1-5-androsten-3β, 7α, 17β?triol, M2-5-androsten-3β, 17β?diol-7-one, and M3-5-androsten-3β, 16α, 17β?triol-7-one were identified as metabolites. As a result, M1 and M2 were endogenous metabolites, and M3 was a new metabolite of 5-androsten-3β-,17β-diol-7-one diacetate. The results of this study on the metabolic pathway and metabolites of the drug (unknown steroid) by LC/MS and GC/MS can be used to determine whether steroid is taken.
목차
CONTENTSChapter 11. Introduction1.1 Introduction 31.2 Metabolism of drugs 41.3 Analysis of metabolites 71.4 The purpose of this study 10Chapter 22. Experiment2.1 Chemicals and reagents 112.2 Instruments 122.3 Experiment methods 132.3.1 Sample preparation 132.3.1.1 Urine sampling 132.3.1.2 Purification 132.3.1.3 Hydrolysis 142.3.1.4 Extraction 152.3.1.5 Derivatization 162.3.2 Validation of Experimental methods 222.3.3 Analysis of 5-androsten-3β-,17β-diol-7-one diacetate and its metabolites using LC/TOF-MS 232.3.4 Analysis of 5-androsten-3β-,17β-diol-7-one diacetate and its metabolites using GC/MSD 23Chapter 33. Results3.1 Separation and extraction of 5-androsten-3β-,17β-diol-7-one diacetate in urine sample 253.1.1 The optimal extraction conditions for metabolites of 5-androsten-3β-,17β-diol-7-one in urine sample 253.1.2 The optimal derivatization conditions of 5-androsten-3β-,17β-diol-7-one metabolites from urine sample 273.1.3 Validation of analysis procedures 303.2 Analysis of 5-androsten-3β-,17β-diol-7-one diacetate and its endogenous metabolites by LC/MS 323.2.1 Identification of M1-5-androsten-3β, 7α, 17β -triol in urine sample 373.2.2 Identification of M2-5-androsten-3β, 17β?diol-7-one in urine sample 373.2.3 Identification of M3-5-androsten-3β, 16α, 17β?triol-7-one in urine sample 383.3 Analysis of 5-androsten-3β-,17β-diol-7-one diacetate and its endogenous metabolites by GC/MSD 383.3.1 Identification of M1-5-androsten-3β, 7α, 17β-triol in urine sample 393.3.2 Identification of M2-5-androsten-3β, 17β?diol-7-one in urine sample 403.3.3 Identification of M3-5-androsten-3β, 16α, 17β?triol-7-one in urine sample 413.4 Investigation of excretion of chemical species using endogenous metabolites of 5-androsten-3,17β-diol-7-one-diacetate 483.5 Investigation of excretion tendencies of metabolites of 5-androsten-3β, 17β?diol-7-one diacetate 50Chapter 44. Conclusion 52References 53
최근 본 자료
댓글(0)
0