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논문 기본 정보

자료유형
학위논문
저자정보

우재훈 (충북대학교, 충북대학교 일반대학원)

지도교수
吳基完
발행연도
2016
저작권
충북대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (2)

초록· 키워드

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Decursinol angelate (DA) is one of the major components of Angelate Gigantis Radix (AGR) and is structurally isomeric decursin. As AGR has been well known that DA inhibits the formation of β-amyloid known as toxins in the brain and reducing the protection of brain cells, it is useful for the prevention and treatment of dementia. This study was performed to confirm whether DA enhances the pentobarbital-induced sleeping behaviors and modulates sleep architectures. Oral administration of DA (10, 25 and 50 mg/kg) markedly suppressed spontaneous locomotor activity. DA prolonged sleeping time, and decreased the sleep latency by pentobarbital in a dose-dependent manner similar to muscimol, both at the hypnotic (42 mg/kg) and sub-hypnotic (28 mg/kg) doses. DA (50 mg/kg) reduced the count of sleep/wake cycles, increased total sleep time, and non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. Additionally, DA increased Cl- influx level in hypothalamic primary cultured neuronal cells of rats. The over-expression of glutamic acid decarboxylase (GAD) and GABAA receptors subtypes were measured. These results suggest that DA would be useful in the treatment of insomnia.

목차

Ⅰ. Introduction 1
1. Decursinol angelate 1
2. Insomnia 3
3. Hypnotics 3
4. γ-Aminobutyric acid 4
5. Hypothalamic cells 7
6. Pentobarbital-induced sleep 7
7. Purpose of this study 8
Ⅱ. Materials and methods 10
1. Reagents and chemicals 10
2. Animals 10
3. Measurement of locomotor activity 11
4. Pentobarbital-induced sleeping behaviors 11
5. Implantation of the EEG telemetric transmitter 12
6. Data analysis 13
7. Primary cell cultures 14
8. Measurement of intracellular chloride influx 15
9. Western blottings of GAD and GABAA receptors subunits 16
10. Statistical analysis 16
Ⅲ. Results 18
1. Decreased effects of DA on locomotor activity in mice 18
2. Effects of DA on the onset and duration of sleep in pentobarbital treated Mice 19
3. Decreased effects of DA on sleep onset of mice treated by subhypnotic dosage of pentobarbital 23
4. Decreased effects of DA (50 mg/kg) on the counts of sleep-wakecycles 25
5. The sleep architectures by DA 27
6. Effects of DA on EEG power density 29
7. Increased effects of DA on chloride influx in primary cultured hypothalamic neuronal cells 32
8. Increased effects of DA on GAD expression 34
9. Increased effects of DA on GABAA receptors subunits expression 36
Ⅳ. Discussion 38
Ⅴ. References 41
Ⅵ. Summary in Korean 48
Ⅶ. Acknowledgements 50

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