지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 서영배
- 발행연도
- 2015
- 저작권
- 대전대학교 논문은 저작권에 의해 보호받습니다.
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Objectives : This study is aimed to evaluate the protective effect of Gastrodiae Rhizoma and steamed, dried & fermented Gastrodiae Rhizoma on LPS-induced hepatic injury in the mice model.
Methods : Mice were randomly divided into 4 groups-Normal group, vehicle group (LPS treated), GR0F0 group (fed GR0F0 - not processed Gastrodiae Rhizoma, before LPS treating) and GR6F4 group (fed GR6F4-fermented with #4 strain and Gastrodiae Rhizoma that steamed and dried 6 times, before LPS treating) with 6 mice in each group.
GR0F0 group and GR6F4 group were fed each material 200 ㎎/㎏/day during 8 days. Then they maintained NPO state for 24 hours. LPS 20 ㎎/㎏ injected to the experimental groups as abdominal injection.
Blood samples were collected 24 hours after LPS injection for liver function and enzyme activity and the livers were harvested for histo- logical, immuno-histochemical and western blot analysis.
Results : The activity was high in order of GR6F4 and GR9F0 (non-fermented Gastrodiae Rhizoma that steamed and dried 9 times) in DPPH radical scavenging activity measurement. In ABTS radical scavenging activity, GR0F0, GR6F4 were a higher order of activity.
Gastrodin was measured a lot in steamed & dried Gastrodiae Rhizoma, gastrodigenin was determined a lot in fermented Gastrodiae Rhizoma. Samples containing the most effective component was GR6F4.
Toxicity was not found in GR0F0 and GR6F4.
Measurement of serum ALT and AST in laboratory animals to induce liver damage by LPS, GR0F0 and GR6F4 showed a significant decrease compared to the vehicle group. As a result of measuring the ROS, GR6F4 showed a significant reduction in both the serum and liver tissues compared to the vehicle group. GR0F0 group showed a significant reduction only in the liver tissues.
AP-1, COX-2, iNOS were significantly decreased GR0F0 group and GR6F4 group. But TNF-α only showed a significant reduction in GR6F4.
Conclusion :
GR0F0, GR6F4 against liver demage in mice with LPS showed significant effects on anti-oxidant and anti-inflammatory action. The effects of GR6F4 showed superior results compared to GR0F0. Therefore, Gastrodiae Rhizoma and steamed, dried & fermented Gastrodiae Rhizoma were thought to have a protective effect on liver injury.
Methods : Mice were randomly divided into 4 groups-Normal group, vehicle group (LPS treated), GR0F0 group (fed GR0F0 - not processed Gastrodiae Rhizoma, before LPS treating) and GR6F4 group (fed GR6F4-fermented with #4 strain and Gastrodiae Rhizoma that steamed and dried 6 times, before LPS treating) with 6 mice in each group.
GR0F0 group and GR6F4 group were fed each material 200 ㎎/㎏/day during 8 days. Then they maintained NPO state for 24 hours. LPS 20 ㎎/㎏ injected to the experimental groups as abdominal injection.
Blood samples were collected 24 hours after LPS injection for liver function and enzyme activity and the livers were harvested for histo- logical, immuno-histochemical and western blot analysis.
Results : The activity was high in order of GR6F4 and GR9F0 (non-fermented Gastrodiae Rhizoma that steamed and dried 9 times) in DPPH radical scavenging activity measurement. In ABTS radical scavenging activity, GR0F0, GR6F4 were a higher order of activity.
Gastrodin was measured a lot in steamed & dried Gastrodiae Rhizoma, gastrodigenin was determined a lot in fermented Gastrodiae Rhizoma. Samples containing the most effective component was GR6F4.
Toxicity was not found in GR0F0 and GR6F4.
Measurement of serum ALT and AST in laboratory animals to induce liver damage by LPS, GR0F0 and GR6F4 showed a significant decrease compared to the vehicle group. As a result of measuring the ROS, GR6F4 showed a significant reduction in both the serum and liver tissues compared to the vehicle group. GR0F0 group showed a significant reduction only in the liver tissues.
AP-1, COX-2, iNOS were significantly decreased GR0F0 group and GR6F4 group. But TNF-α only showed a significant reduction in GR6F4.
Conclusion :
GR0F0, GR6F4 against liver demage in mice with LPS showed significant effects on anti-oxidant and anti-inflammatory action. The effects of GR6F4 showed superior results compared to GR0F0. Therefore, Gastrodiae Rhizoma and steamed, dried & fermented Gastrodiae Rhizoma were thought to have a protective effect on liver injury.
목차
Ⅰ. 서 론 1Ⅱ. 재료 및 방법 21. 재료 21) 약재 22) 실험 동물 23) 시약 24) 기기 32. 방법 41) 시료제조 4(1) 증숙 4(2) 발효 62) 항산화 활성 측정과 성분 분석 8(1) DPPH radical scavenging activity 측정 8(2) ABTS radical scavenging activity 측정 8(3) 성분분석 93) LPS에 의한 급성 간손상 개선 효능평가 10(1) 급성 간손상 모델 및 약물 투여 10(2) 체중, 식이섭취량, 식이효율 측정 10(3) 혈청의 ALT, AST 측정 11(4) ROS 측정 11(5) Western blot analysis 11(6) 조직학적 분석 124) 통계처리 12Ⅲ. 결과 131. pH 측정 132. 항산화 활성 및 성분분석 141) DPPH radical scavenging activity 142) ABTS radical scavenging activity 153) 성분분석 16(1) 표준품 HPLC 분석 16(2) 성분 정량 분석 17(3) 성분 함량 분석 184) 최종 시료 선정 193. LPS에 의한 간손상에 대한 간보호 효능 측정 201) 체중 및 식이효율의 변화 202) 간의 질량 비율 213) 혈청의 ALT, AST 평가 224) ROS 측정 235) 간 조직의 염증성 cytokine 및 매개인자 측정 24(1) AP-1, NF-κB p65 측정 24(2) COX-2, iNOS, TNF- 측정 256) 조직학적 분석 26Ⅳ. 고 찰 27Ⅴ. 결 론 33참고문헌 35Abstract 41
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