This study is an experimental research to verify the effects of “Kamisipjeondaebotang” in fracture healing. I tried to investigate its healing effects through the blood analysis and radiation measurement with a fractured skull mouse model. The results were as follows :
1. According to the measurement results of heavy metal content in GST, cadmium was detected below the reference value, and toxic materials such as lead, arsenic and mercury were not detected at all.2. GST treatment group showed no toxic infectionsin a liver-function examination AST, ALT and renal-function examination Cr, and BUN . The test results were within the normal range and no toxicity was induced.3. When I measured a cell toxicity in RAW 264.7 cells which were treated with GST, I could not find any toxicity in them.4. GST showed an increased radical scavenging ability in concentration -dependent manner, in the influence on DPPH, ABTS radical scavenging ability.5. The level of the serums, such as osteocalcin, calcitonin, CTXⅡ, TGF-β, BMP-2, insulin ALP and PGE2, which have effect on fracture healing in GST treated group, showed a high activity compared to the control group. 6. An X-RAY inspection of the GST treated group which was conducted 4 and 8 weeks after the fracture showed that the fracture healing process is going visibly faster than that of the control group. 7. After the test, BMD measurement showed the results of 0.59±0.01 ㎎/㎠ in the control group, 0.67±0.05 ㎎/㎠ in GST 200 mg/kg treated group, and 0.64±0.02 ㎎/㎠ in GST 400 mg/kg treated group, which indicates there is an increase compared to the control group.8. After the test, I conducted a comparative analysis on the micro-CT Test results of the GST treatment group and the control group. The analysis indicated the result of 3.67±0.70 U^3 in the control group, 6.38 ± 0.70 U ^ 3 in GST 200 ㎎ / ㎏ treated group and 4.99±0.71 U^3 in GST 400 ㎎ / ㎏ treated group, This demonstrates that there is a meaningful increase in the treated group compared to the control group. (** : p<0.01, * : p<0.05)

Based on the above test results, I would say that GST has some effective capability in new bone formation and bone mineral density, when it is applied to a skull fractured mouse model. I expect that we can apply GST in curing fractures through a comprehensive clinical studies.