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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김영철
- 발행연도
- 2015
- 저작권
- 경희대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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Objectives : This study was performed to investigate the anti-cancer
effects of Rhus verniciflua Stokes extracted with sterile distilled water on
Cholangiocarcinoma cell lines.
Materials and Methods : Two Cholangiocarcinoma Cell Lines, SNU-1079
and SNU-1196, were used in this study. Cells were treated with different
concentrations of Rhus verniciflua Stokes for 24, 48, 72 hours. Cell count,
viability, apoptosis and mRNA expressions of Bax, Bcl-2, Mcl-1, Survivin,
Caspase-3, Cyclin D1, P21 were determinded by automatic cell counter
(ADAM-MC), MTT assay, apoptosis assay (Annexin-V/PI staining) and
RT-PCR.
Result : All cells treated with Rhus verniciflua Stokes showed decreased cell counting with dose-dependent manner. Rhus verniciflua Stokes inhibited proliferation of SNU-1196 with dose-dependent manner, but SNU-1079 proliferation was inhibited in long-time culture group with dose-dependent manner. The proportion of early and late stage apoptotic cells was increased by Rhus verniciflua Stokes with dose-dependent manner in SNU-1196. In contrast, it was increased significantly in SNU-1079 treated with high-dose Rhus verniciflua Stokes. After the treatment of Rhus verniciflua Stokes, the mRNA expression of Bcl-2 was decreased while Bax was increased in SNU-1079. Cyclin D1 mRNA levels were decreased in SNU-1196 with dose-dependent manner. P21 expression was increased in all cells after the treatment of Rhus verniciflua Stokes.
Conclusion : Rhus verniciflua Stokes appears to have more potential as a therapeutic agent of Cholangiocarcinoma.
effects of Rhus verniciflua Stokes extracted with sterile distilled water on
Cholangiocarcinoma cell lines.
Materials and Methods : Two Cholangiocarcinoma Cell Lines, SNU-1079
and SNU-1196, were used in this study. Cells were treated with different
concentrations of Rhus verniciflua Stokes for 24, 48, 72 hours. Cell count,
viability, apoptosis and mRNA expressions of Bax, Bcl-2, Mcl-1, Survivin,
Caspase-3, Cyclin D1, P21 were determinded by automatic cell counter
(ADAM-MC), MTT assay, apoptosis assay (Annexin-V/PI staining) and
RT-PCR.
Result : All cells treated with Rhus verniciflua Stokes showed decreased cell counting with dose-dependent manner. Rhus verniciflua Stokes inhibited proliferation of SNU-1196 with dose-dependent manner, but SNU-1079 proliferation was inhibited in long-time culture group with dose-dependent manner. The proportion of early and late stage apoptotic cells was increased by Rhus verniciflua Stokes with dose-dependent manner in SNU-1196. In contrast, it was increased significantly in SNU-1079 treated with high-dose Rhus verniciflua Stokes. After the treatment of Rhus verniciflua Stokes, the mRNA expression of Bcl-2 was decreased while Bax was increased in SNU-1079. Cyclin D1 mRNA levels were decreased in SNU-1196 with dose-dependent manner. P21 expression was increased in all cells after the treatment of Rhus verniciflua Stokes.
Conclusion : Rhus verniciflua Stokes appears to have more potential as a therapeutic agent of Cholangiocarcinoma.
목차
Ⅰ. 緖論 1Ⅱ. 本論 21. 재료 및 방법 22. 세포수 분석 33. 세포활성도 분석 (MTT assay) 34. 세포자멸 분석 (Annexin-V/PI assay) 35. 역전사 중합효소 연쇄반응 분석 (RT-PCR) 4Ⅲ. 結果 61. 漆皮 추출물이 담도암 세포주 세포수에 미치는 영향 62. 漆皮 추출물이 담도암 세포주 세포증식에 미치는 영향 73. 漆皮 추출물이 담도암 세포주 세포자멸에 미치는 영향 84. 漆皮 추출물에 의한 Bcl-2, Bax, Mcl-1 mRNA 발현의 변화 115. 漆皮 추출물에 의한 Survivin, Caspase-3 mRNA 발현의 변화 166. 漆皮 추출물에 의한 Cyclin D1, P21 mRNA 발현의 변화 19Ⅳ. 考察 22Ⅴ. 結論 25Ⅵ. 參考文獻 26ABSTRACT 29
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