Acid mine drainage occurrence is a serious environmental problem by mining industry, it usually contains high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of the greatest concern. Microorganisms can mediate the formation of minerals by a biomineralization process. Microbially induced calcite precipitation (MICP) has proven to be an effective means to capture heavy metals. Microbial processes that bind metals and form minerals are widespread and represent a fundamental part of key biogeochemical cycles. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a mine-impacted area. All isolated bacteria were identified as Sporosarcina pasteurii with more than 98% of similarity by analysis of 16S rRNA gene sequence. Therefore, the isolated bacteria were named Sporosarcina sp. KM-01 (KP000029), KM-07 (KP000030), and KM-12 (KP000031). The KM-01, KM-07, and KM-12 strains were shown to be able to hydrolyze urea. When the production of ammonium ion by isolated bacteria was investigated by urease activity analysis of indophenol method, the strain KM-01, KM-07 and KM-12 efficiently convert urea to ammonium ion with the yield of 0.70 mM, 0.64 mM, 0.53 mM, respectively. The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS and XRD analysis showed that calcium carbonate was successfully produced and increased with time. Column leaching experiment was performed to determine whether MICP serves as barrier to resist diffusion from the heavy metal contaminated soil. Metal concentrations in the leachates decreased with respect to time in the treated columns with bacterial isolates. These findings demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals. These results also suggest that three bacterial isolates may be used in the bioremediation of heavy metal contaminated mine soils as a practical example of biomineralization processes.