지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 안상낙
- 발행연도
- 2015
- 저작권
- 충남대학교 논문은 저작권에 의해 보호받습니다.
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Ammonium (NH4+) is the major source of nitrogen for rice growth and developments (Oryza sativa L.). The objectives of this study were to examine NH4+ mediated five traits response; shoot length (SL), shoot weight (SW), root length (RL), root weight (RW), and root dry weight (RDW) of early rice seedlings grown in hydroponic condition. Also to identify putative QTLs associated with enhanced five traits under different NH4+ levels. All of the seedlings were grown for 11days in hydroponic condition with the 0, 250, 500μM NH4+ levels. The seedlings of rice were grown in growth chamber, also. To establish the appropriate material under different NH4+ we carried out among nine type of rice, and Moroberekan and O.rufipogon did not show significant difference for four traits in various NH4+ levels. Therefore, we selected Moroberekan. When grown in different NH4+ levels, rice plants at 250uM NH4+ level showed the maximum growth compared to 0μM NH4+ levels. In contrast, 500μM NH4+ levels produced NH4+ toxicity and poor plant growth. Hence, 250μM NH4+ was set as the optimum nitrogen concentration. 40 chromosome segment substitution lines (CSSLs) derived from a cross between the rice varieties ‘Ilpumbyeo’ and ‘Moroberekan’ were evaluated for five traits related to various NH4+ levels. The CSSL parents had contrasting shoot and root response to NH4+ levels. Five traits of Moroberekan were insensitive to NH4+ levels, whereas Ilpumbyeo was responsive.
In conclusion, this work is only a starting point for characterizing the genetic basis of rice growth in different NH4+ levels. QTL regions that were found in our studies would be useful in developing nitrogen-use efficiency (NUE) lines in the breeding program.
In conclusion, this work is only a starting point for characterizing the genetic basis of rice growth in different NH4+ levels. QTL regions that were found in our studies would be useful in developing nitrogen-use efficiency (NUE) lines in the breeding program.
목차
INTRODUCTION 1MATERIALS AND METHODS 4Plant materials 4Seeds sterilization 4Hydroponic condition 5Evaluation of traits 5Data analysis 6RESULTS 7Variation of five traits in response to NH4+ supply among rice accessions 7Frequency distribution of 40 CSSLs and parents in five traits 16QTL analysis for five traits of seedlings 23DISCUSSION 28REFFERENCE 33KOREAN SUMMARY 39
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