Abstract

1. Objectives
To evaluate the neuroprotective effects of modified Yuldahanso-tang (MYH), a Chinese traditional medicinal prescription in a Parkinson''s disease mouse model.

2. Methods
1) Four groups (each of 8 rats per group) were used in this study.
2) The neuroprotective effect of MYH was examined in a Parkinson''s disease mouse model. C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days.
3) To measure the amount of dopamine in mice brain, striatum- substantia nigra were examined by Bradford.
4) Immunohistochemistry was examined in the MPTP-induced Parkinson''s disease mice to evaluate the neuroprotective effects of MYH on ST and SNpc.
5) Ischemic mice brain stained with TTC in the MPTP-induced Parkinson''s disease to find out ischemia and tissue damage in mouse
6) In order to investigate the effect of MYH on recovery of memory , we examined the memory by using Morris water maze test.
7) Immunohistochemistry was examined in the MPTP-induced Parkinson''s disease mice to evaluate the neuroprotective effects of MYH on hippocampal lesion.
8) In order to investigate the effect of MYH on recovery of behavioral deficits, we examined the motor function by using and Forced swimming test.
9) The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of Neurotransmitters in MPTP-MYH group.
3. Results and Conclusion
This experiment suggests that the neuroprotective effect of MYH is mediated by the increasa in Dopamin, TH-ir cell, Hippocampus and Serotonin. Furthermore, MYH essential oil may serve as a potential preventive or therapeutic agent regarding Parkinson''s disease.


Key words : modified Yuldahanso-tang (MYH), Parkinson’s disease, Dopamin, TH-ir cell, Hippocampus, Serotonin.