지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김선민
- 발행연도
- 2015
- 저작권
- 인하대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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In this research, we developed an in vitro 2D skin model for pathological assay of dermal wound healing.
Firstly, nano-grooved patterns were used to mimic aligned fibroblast tissue and dermal environments. Various nano-grooved patterns in this research were fabricated with biocompatible PDMS (polydimethylsiloxane) polymer using capillary force lithography and the pattern ratios of each pattern were 1:1, 1:2, 1:5 which have equal width (550 nm) and depth (325nm). Dermal fibroblast cells were cultured on various surfaces including negative control which is non-patterned surface. Because dermal fibroblasts were aligned on collagen fiber bundles in dermis, we analyzed the cellular growth and behavior as a alignment index to form a well mimicked tissue. The cells on the higher ratio of grooved surfaces were well-aligned to pattern direction and more fibrous shape. Moreover, cell migration on the grooved pattern of 1:2 ratio was the fastest compared to on the other patterns.
Secondly, wound generation was well-mimicked using laminar flow of Trypsin-EDTA solution in microfluidic skin model integrated with nano-grooved patterns and then cellular behavior during wound healing process was analyzed in this skin model.
This research can provide a standard pathological assay platform for cutaneous wound healing and information for understanding skin tissue.
Firstly, nano-grooved patterns were used to mimic aligned fibroblast tissue and dermal environments. Various nano-grooved patterns in this research were fabricated with biocompatible PDMS (polydimethylsiloxane) polymer using capillary force lithography and the pattern ratios of each pattern were 1:1, 1:2, 1:5 which have equal width (550 nm) and depth (325nm). Dermal fibroblast cells were cultured on various surfaces including negative control which is non-patterned surface. Because dermal fibroblasts were aligned on collagen fiber bundles in dermis, we analyzed the cellular growth and behavior as a alignment index to form a well mimicked tissue. The cells on the higher ratio of grooved surfaces were well-aligned to pattern direction and more fibrous shape. Moreover, cell migration on the grooved pattern of 1:2 ratio was the fastest compared to on the other patterns.
Secondly, wound generation was well-mimicked using laminar flow of Trypsin-EDTA solution in microfluidic skin model integrated with nano-grooved patterns and then cellular behavior during wound healing process was analyzed in this skin model.
This research can provide a standard pathological assay platform for cutaneous wound healing and information for understanding skin tissue.
목차
1. Introduction 12. Materials and methods 52.1 Cell culture and mimicking in vitro tissue on nano-grooved pattern surfaces 52.1.1 Materials and reagents 52.1.2 Cell culture 62.1.3 Analysis of cellular growth 72.1.4 Analysis of cellular behavior 72.1.5 Immunostaining assay 82.2 Development of microfluidic skin model for wound healing assay 92.2.1 Fabrication of micro-channel using soft lithography method 92.2.2 Fabrication of microfluidic system integrated nano-grooved pattern surfaces 102.2.3 Cell culture 102.2.4 Wound generation using laminar flow of chemical 112.2.5 Wound healing assay 113. Results 123.1 Analysis of cellular growth and behavior on nano-grooved pattern surfaces 123.1.1 Analysis of nano-grooved pattern surfaces 123.1.2 Analysis of cellular growth shape 133.1.3 Analysis of cellular migration 143.1.4 Immunostaining assay 143.2 In vitro wound healing assay 153.2.1 Microfluidic 2D skin model integrated nano-grooved pattern surface 153.2.2 In vitro mimicry about in vivo dermal layer 153.2.3 Wound healing assay in 2D skin model 164. Conclusions 18References 30
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