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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 정귀택
- 발행연도
- 2015
- 저작권
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Abstract
In chapter 1, to evaluate the efficacy of marine macro-algae Enteromorpha intestinalis as a potential bioenergy resource, the effects of reaction conditions (solid-to-liquid ratio, reaction temperature, and reaction time) on sugars produced by a combined process of hydrothermal and enzymatic hydrolysis were investigated. As a result of the hydrothermal hydrolysis, a 7.3 g/L (8% yield) total reducing sugar was obtained under conditions including solid-to-liquid ratio of 1:10, reaction temperature of 170°C, and reaction time of 60 min. By subsequent (post-hydrothermal) enzymatic hydrolysis of samples treated at 170°C for 30 min, a 20.1 g/L (22% yield) was achieved.
In chapter 2, to evaluate antioxidant activities of E. intestinalis, three solvents (hexane, chloroform and methanol) were applied to obtain extracts. The extraction yields are hexane (A) 1.11%, chloroform (B) 0.94%, and methanol (C) 8.2%. Also, the contents of total phenolic compounds of extract A, B, C are 4.03%, 8.15%, and 2.33%, respectively. In the results of antioxidant activity, extract A and B present the higher DPPH activity than that of control (BHA and ascorbic acid). The DPPH radical scavenging activity and SOD-like activity are ordered as B>C>A. However, extract C show high value in ferric reducing antioxidant power assay. In nitrite scavenging activity, extract A is relatively higher than others. However, the antioxidant activities are lower than that of controls (ascorbic acid and BHA). The antioxidant activities are presented the increasing pattern of increasing by the increasing of extract concentration.
In chapter 1, to evaluate the efficacy of marine macro-algae Enteromorpha intestinalis as a potential bioenergy resource, the effects of reaction conditions (solid-to-liquid ratio, reaction temperature, and reaction time) on sugars produced by a combined process of hydrothermal and enzymatic hydrolysis were investigated. As a result of the hydrothermal hydrolysis, a 7.3 g/L (8% yield) total reducing sugar was obtained under conditions including solid-to-liquid ratio of 1:10, reaction temperature of 170°C, and reaction time of 60 min. By subsequent (post-hydrothermal) enzymatic hydrolysis of samples treated at 170°C for 30 min, a 20.1 g/L (22% yield) was achieved.
In chapter 2, to evaluate antioxidant activities of E. intestinalis, three solvents (hexane, chloroform and methanol) were applied to obtain extracts. The extraction yields are hexane (A) 1.11%, chloroform (B) 0.94%, and methanol (C) 8.2%. Also, the contents of total phenolic compounds of extract A, B, C are 4.03%, 8.15%, and 2.33%, respectively. In the results of antioxidant activity, extract A and B present the higher DPPH activity than that of control (BHA and ascorbic acid). The DPPH radical scavenging activity and SOD-like activity are ordered as B>C>A. However, extract C show high value in ferric reducing antioxidant power assay. In nitrite scavenging activity, extract A is relatively higher than others. However, the antioxidant activities are lower than that of controls (ascorbic acid and BHA). The antioxidant activities are presented the increasing pattern of increasing by the increasing of extract concentration.
목차
목 차List of Figures and Table ⅢAbstract ⅤChapter Ⅰ열수 및 효소 가수분해를 통한 거대녹조류 창자파래로부터 환원당 생산1. 서론 22. 재료 및 방법 52.1 실험 재료 52.2 회분식 열수 가수분해 52.2.1 고액비의 영향 62.2.2 온도의 영향 62.3 효소 가수분해 62.4 엄격계수 62.5 분석방법 73. 결과 및 고찰 83.1 열수 가수분해를 통한 환원당 생성 83.1.1 고액비의 영향 83.1.2 반응온도의 영향 113.2 효소 가수분해에 대한 열수처리의 영향 143.3 엄격계수의 영향 174. 결론 18참고문헌 19Chapter Ⅱ거대녹조류 창자파래 추출물의 항산화 활성 평가1. 서론 252. 재료 및 방법 272.1 실험재료 272.2 추출 및 분획 272.3 항산화 활성 측정 272.3.1 DPPH radical 소거능 282.3.2 환원력 (Ferric reducing antioxidant power (FRAP) assay) 282.3.3 Superoxide dismutase (SOD) 유사활성 292.3.4 아질산 소거능 292.4 총 페놀성 화합물의 함량 293. 결과 및 고찰 313.1 추출 및 분획 313.2 총 페놀성 화합물의 함량 333.3 항산화 활성 353.3.1 DPPH radical 소거능 353.3.2 환원력 (FRAP assay) 373.3.3 Superoxide dismutase (SOD) 유사활성 393.3.4 아질산 소거능 414. 결론 43참고문헌 44감사의 글 47
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