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논문 기본 정보

자료유형
학위논문
저자정보

허윤지 (충남대학교, 忠南大學校 大學院)

지도교수
이기택
발행연도
2014
저작권
충남대학교 논문은 저작권에 의해 보호받습니다.

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Enzymatic interesterification and acyl migration products and blending compounds for low saturated level of hydrogenated coconut oil (HCO) were produced by fractionated palm stearin, high oleic sunflower oil (HOSO), acyl migration palm mid fraction (A PMF) and HCO. Palm stearin solid (PSS) and palm stearin liquid (PSL) were concentrated from palm stearin using acetone fractionation at 25℃ for 24 hours. PSS and HOSO were used for lipase-catalyzed interesterificaiton with Lypozyme RMIM in shaking water bath at 60℃ and 185 rpm for 6 hours. To reduce monoacylglycerol (MAG), diacylglycerol (DAG) and free fatty acid (FFA) in reactants, silica gel and florisil column were applied. Products Ⅰ was obtained after 2-steps acetone fractionation. PSL was blended with HCO (products Ⅱ). Asymmetric 1, 2-dipalmitoyl-3-oleoyl-glycerol (PPO) triacylglycerol (TAG) was produced from PMF by acyl migration with Lypozyme TLIM. It was performed for different reaction time (3, 6, 9 hours) and 185 rpm at 80℃ in shaking water bath. After acetone fractionation to remove high melting TAG (ex. PPP), they were blended with HCO (product Ⅲ, Ⅳ and Ⅴ according to reaction time). In fatty acid composition analysis by gas chromatography (GC), it confirmed that oleic acid (C18:1) in PSL was 39.5 area% in TAG and increased 66.5 area% in sn-2 position. From sn-2 position fatty acid composition, sum of palmitic acid was increased (57.0∼61.3 area%) in A PMFs. However they were no significant different on reaction time. The major TAG species were analyzed by reverse-phased HPLC. HCO and crude fat from coffee cream (coffee cream) were similarly distributed in ranged from PN value 32∼42 (ex. CaCaLa, CaCaM/CaLaLa, LaLaLa, LaLaM, LaMM). product Ⅰ was POO/OPO (7.9 area%) and POP/PPO (69.7 area%). Product Ⅱ was contained mixture of HCO and PSL TAG forms. Products Ⅲ, Ⅳ and Ⅴ from A PMF and HCO were spread both TAG species. According to differential scanning calorimetry (DSC), transition peaks of HCO (26.10℃) and coffee cream (26.72℃) were resemble. However melting and crystallization behavior of product Ⅰ were different form HCO. Melting point of product Ⅱ (23.47℃) was lower than HCO even though melting curve was similar with HCO. Product Ⅲ, Ⅳ and Ⅴ melting points were higher than product Ⅱ and less than HCO because of eutectic interaction. On the solid fat index (SFI), except for product Ⅰ(enzymatic product), blending compounds (product Ⅱ, Ⅲ, Ⅳ and Ⅴ) were similar to HCO and coffee cream with sharp decrease solid fat contents and relatively narrow melting point range from 36 ∼ 43℃. On the other hand, It was confirmed that slip melting point of product Ⅱ was lower than other products, HCO and coffee cream. Especially, the saturated fatty acid contents on blending compounds were less 20% compared to the HCO and coffee cream. Also, emulsion stability remained stable over 20 days.

목차

Ⅰ. 서 론 1
Ⅱ. 재료 및 실험방법 5
1. 실험 재료 및 시약 5
2. 산가측정 및 과산화물제거 5
3. 팜스테아린의 용매 분별 6
4. 효소적 interesterification 반응 8
4.1 효소적 interesterification 반응물의 정제 및 용매분별 8
5. 팜스테아린 액상유(PSL)와 야자경화유(HCO)의 blending 9
6. 팜중부유(PMF)의 acyl migration 반응과 정제 및 용매분별 9
6.1. Acylmigration 반응물과 야자경화유의 blending 10
7. 산분해법에 의한 조지방 추출 10
8. 지방산 조성 분석 11
9. Pancreatic lipase를 이용한 가수분해 후 위치별 지방산 조성 분석 11
10. RP-HPLC를 이용한 triacylglycerol(TAG)의 조성 분석 12
11. Differential scanning calorimetry(DSC) 분석 13
12. Melting point 측정 13
13. 지방산 정량 14
14. 유화액 제조 및 지방구 크기 측정 15
15. 현미경 관찰 및 크리밍 안정성 변화 측정 18
16. 통계처리 18
Ⅲ. 결과 및 고찰 19
1. 지방산 조성 및 위치별 지방산 조성 19
1.1 팜스테아린 분별물 19
1.2 Acyl migration반응을 한 팜중부유(A PMF) 22
2. 효소적 interesterification 반응물들의 TAG 조성 분석 25
3. 커피크림 조지방, 야자경화유, blending 반응물의 TAG 조성 분석 27
4. Acyl migration PMF blending 반응물들의 TAG 조성 분석 29
5. DSC를 이용한 흡열 및 발열곡선 분석 31
5.1 효소적 interesterification 반응물 31
5.2 커피크림 조지방, 야자경화유, 팜스테아린 액상유(PSL) blending 반응물 34
5.3 Acyl migration 팜중부유(PMF) blending 반응물 37
6. Solid fat index(SFI), slip melting point 및 complete melting point 분석 40
7. 지방산 함량 정량 분석 44
8. 유화 특성 46
Ⅳ. 결 론 51
Ⅴ. 참고문헌 53
ABSTRACT 57

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