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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 백혜자
- 발행연도
- 2014
- 저작권
- 부경대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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Prostaglandins (PGs), arachidonic acid (AA) and cyclooxygenase (COX) metabolites, and pH of the incubation medium have been implicated in final maturation and ovulation in teleost.
The purpose of this studies was to investigate the effect of prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and medium pH on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation of the marine fish, Chasmichthys dolichognathus. The oocytes of 0.80, 0.84, 0.89 and 0.94 mm were incubated with 5, 50 and 500 ng/ml of PGE2 and PGF2α for 24 hours. And the oocytes of 0.84, 0.91 and 0.95 mm were incubated with 7.2, 7.4, 7.7, 8.0 and 8.3 medium pH for 24~36 hours. In the oocytes of 0.84 mm and 0.94 mm, PGE2 significantly induced on GVBD at 50 ng/ml compare with control but PGF2α had no significant effect on GVBD and ovulation. In the oocytes of 0.80 mm and 0.89 mm, PGE2 and PGF2α had no significant effect on GVBD and ovulation at the concentration tested. No significant differences on GVBD and ovulation were observed in medium pH levels. The results suggest that PGE2 may induce GVBD at 50 ng/ml.
The purpose of this studies was to investigate the effect of prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and medium pH on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation of the marine fish, Chasmichthys dolichognathus. The oocytes of 0.80, 0.84, 0.89 and 0.94 mm were incubated with 5, 50 and 500 ng/ml of PGE2 and PGF2α for 24 hours. And the oocytes of 0.84, 0.91 and 0.95 mm were incubated with 7.2, 7.4, 7.7, 8.0 and 8.3 medium pH for 24~36 hours. In the oocytes of 0.84 mm and 0.94 mm, PGE2 significantly induced on GVBD at 50 ng/ml compare with control but PGF2α had no significant effect on GVBD and ovulation. In the oocytes of 0.80 mm and 0.89 mm, PGE2 and PGF2α had no significant effect on GVBD and ovulation at the concentration tested. No significant differences on GVBD and ovulation were observed in medium pH levels. The results suggest that PGE2 may induce GVBD at 50 ng/ml.
목차
Ⅰ. 서론 1Ⅱ. 재료 및 방법 41. 실험어 42. 난모세포 배양 43. 난모세포의 최종 성숙(GVBD)과 배란 유무 관찰 74. 통계분석 7Ⅲ. 결과 81. 난모세포의 조직형태학적 관찰 82. Prostaglandins(PGs)과 pH가 난모세포의 GVBD와 배란에 미치는 영향 121) PGs 효과 122) pH 효과 16Ⅳ. 고찰 18Ⅴ. 요약 22Ⅵ. 참고문헌 23Ⅶ. 감사의 글 29
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