목차

Ⅰ. 서론
1. 차가버섯의 기능성 1
2. 반응표면분석법 5
3. 연구의 목적 6
Ⅱ. 실험 재료 및 방법
1. 실험 시약 7
2. 추출 건조물 제조 7
3. 총 폴리페놀 함량 측정 8
4. 항산화능 측정
4.1 ABTS 라디컬 소거능 9
4.2 Oxygen radical absorbance capacity (ORAC) 10
5. Glucan류 함량 측정
5.1 총 glucan과 β-glucan의 함량 측정 11
5.2 β-1,3-Glucan의 함량 측정 12
6. Betulinic acid의 측정 12
7. 반응표면분석법 실험 계획 및 데이터의 분석 13
8. 반응표면모델의 검증 17
Ⅲ. 실험 결과 및 고찰
1. 추출 수율 18
2. 총 폴리페놀 함량 23
3. 항산화능
3.1 ABTS 라디컬 소거능 27
3.2 ORAC 31
4. Glucan류
4.1 총 glucan과 β-glucan 35
4.2 β-1,3-Glucan 37
5. Betulinic acid 41
6. 최적추출조건의 결정 및 모델의 검증 42
Ⅳ. 요약 및 결론 47
참고 문헌 49
Abstract 53
List of Table
Table 1. Import of chaga mushroom in Korea (January 2007 to December 2011) 3
Table 2. Independent variables and their coded and actual values used for optimization of extraction condition from chaga mushroom 15
Table 3. Experimental order of central composite design used for response surface methodology with three independent variables from chaga mushroom 16
Table 4. Experimental data of extraction yield for optimization of extraction condition from chaga mushroom 19
Table 5. Statistical analysis of central composite design for extraction yield from chaga mushroom 20
Table 6. Experimental data of total phenolics for optimization of extraction condition from chaga mushroom 24
Table 7. Statistical analysis of central composite design for total phenolics from chaga mushroom 25
Table 8. Experimental data of ABTS for optimization of extraction condition from chaga mushroom 28
Table 9. Statistical analysis of central composite design for ABTS from chaga mushroom 29
Table 10. Experimental data of ORAC for optimization of extraction condition from chaga mushroom 32
Table 11. Statistical analysis of central composite design for ORAC from chaga mushroom 33
Table 12. Experimental data of β-1,3-glucans for optimization of extraction condition from chaga mushroom 38
Table 13. Statistical analysis of central composite design for β-1,3-glucans from chaga mushroom 39
Table 14. Optimum conditions and comparison of predicted and observed values for verification 45
Table 15. Conditions to optimize the extractions of bioactive compounds (total phenolics and β-1,3-glucans) and 5 response variables (yield, total phenolics, ABTS, ORAC and β-1,3-glucans) 46
List of Figure
Figure 1. Response surface plots and contour plots showing the effects of ethanol concentration (X1) and temperature (X2) on extraction yield (Y1) from chaga mushroom 21
Figure 2. Response surface plots and contour plots showing the effects of ethanol concentration (X1) and temperature (X2) on total phenolics (Y2) from chaga mushroom 26
Figure 3. Response surface plots and contour plots showing the effects of ethanol concentration (X1) and temperature (X2) on ABTS (Y3) from chaga mushroom 30
Figure 4. Response surface plots and contour plots showing the effects of ethanol concentration (X1) and temperature (X2) on ORAC (Y4) from chaga mushroom 34
Figure 5. Response surface plots and contour plots showing the effects of ethanol concentration (X1) and temperature (X2) on β-1,3-glucans (Y5) from chaga mushroom 40