Myxobacteria are Gram-negative soil bacteria, production a large number of bioactive substances, and have been found more than 500 bioactive substances. Myxococcus species the most separated in koera.
I culture extracts of 217 Myxococcus strains isolated in Korea, compared their antimicrobial activity against Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. As a result, 2 strains of M. virescens showing antibacterial activity against P. aeruginosa, and antifungal activity against Candida albicans showing more than 54% in Myxococcus species(115 of 217 strains).
5 strains of Myxococcus species distinguish 16S rDNA sequences and the shapes of their fruiting bodies, the selected geographical origin and bioactive substances. The culture extracts produced from each strains, and get the result of two by high-performance liquid chromatography(HPLC). Firstly, production of substances peak was difference according to strains. Secondly, the same strains was similar in correlation between phylogenetic tree and substances peak. Production of substances of M. stipitatus KYC4013 was found antifungal substance melithiazol and antiviral substance phenalamide by high-resolution mass spectrometry(HR-MS). The substances was separate 60~80% gradient elution of acetonitrile, and the mostly produce in CYS medium for more than 7 days.
Also make use of M. stipitatus KYC577 was included antifungal activity and fluorescent pigment is made M. stipitatus KYC577-F01 and F02 by mariner transposon. M. stipitatus KYC577-F01 gene cluster analysis by PCR was inserted Gene locus STAUR_2580, predicted uncharacterized protein. M. stipitatus KYC577-F01 by HPLC analysis was separate not the fluorescent pigment but another substances. In the same way, phenalamide is cloning unknown gene cluster, but result is not what want.