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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 발행연도
- 2014
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이 논문의 연구 히스토리 (2)
2014
Hepatoprotective evaluation of ganoderma lucidum pharmacopuncture : Ethanol induced acutely and chronically liver injury in vivo studies
장선희
韓醫學科
2014.01
학위논문
Hepatoprotective Evaluation of Ganoderma lucidum Pharmacopuncture: In vivo Studies of Ethanol-induced Acute Liver Injury
장선희
,
조성우
,
윤현민
외 3명
Journal of Pharmacopuncture
2014.01
학술저널
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초록· 키워드
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This study is aimed at investigating the protective effect of Ganoderma lucidum pharmacopuncture (GLP) on hepatotoxicity induced acutely and chronically by ethanol (EtOH) in rats.
SD Rats were divided into 4 groups of 8 animals each: normal, control, NP (Normal saline pharmacopuncture) and GLP. Control, NP and GLP groups received ethanol (acute & chronic). NP and GLP groups were daily treated with injection of normal saline and Ganoderma lucidum extract respectively. Control group received no treatment.
As a result, GLP reduced the histological changes induced by EtOH in both acute and chronic liver injury. GLP significantly reduced activities of ALT enzymes increase induced by EtOH for 6h. In test for 4 weeks of EtOH administration, both AST and ALT enzymes of GLP group were significantly reduced. GLP significantly ameliorated SOD and CAT activity in both acute and chronic injury. And GLP significantly decreased BAX and increased Bcl-2 immunoreactivity expression in chronic liver injury.
In conclusion, the present study indicated the protective efficacy of GLP treatment against EtOH-induced hepatic injury in SD rats by modulating ethanol metabolizing enzymes activity, attenuating oxidative stress, and inhibiting mitochondrial damage-mediated apoptosis.
SD Rats were divided into 4 groups of 8 animals each: normal, control, NP (Normal saline pharmacopuncture) and GLP. Control, NP and GLP groups received ethanol (acute & chronic). NP and GLP groups were daily treated with injection of normal saline and Ganoderma lucidum extract respectively. Control group received no treatment.
As a result, GLP reduced the histological changes induced by EtOH in both acute and chronic liver injury. GLP significantly reduced activities of ALT enzymes increase induced by EtOH for 6h. In test for 4 weeks of EtOH administration, both AST and ALT enzymes of GLP group were significantly reduced. GLP significantly ameliorated SOD and CAT activity in both acute and chronic injury. And GLP significantly decreased BAX and increased Bcl-2 immunoreactivity expression in chronic liver injury.
In conclusion, the present study indicated the protective efficacy of GLP treatment against EtOH-induced hepatic injury in SD rats by modulating ethanol metabolizing enzymes activity, attenuating oxidative stress, and inhibiting mitochondrial damage-mediated apoptosis.
목차
I. Introduction 1II. Materials and methods 41. Acute hepatotoxicity1-1. Preparation of solution 41-2. Animals and treatment 41-3. Histopathological analysis 51-4. Assessment of liver function 51-5. Antioxidant enzyme activity assays 61-6. Statistical analysis 72. Chronic hepatotoxicity2-1. Preparation of solution 82-2. Animals and treatment 82-3. Histopathological analysis 82-4. Assessment of liver function 92-5. Antioxidant enzyme activity assays 92-6. Immunohistochemistry 102-7. Statistical analysis 10III. Results 111. Effect of GLP on acute liver injury1-1. Histopathological findings of liver tissue in acute injury 111-2. Liver functions 131-3. Liver antioxidant enzyme activities 162. Effect of GLP on chronic liver injury2-1. Body weight 192-2. Histopathological findings of liver tissue in chronic injury 212-3. Liver functions 232-4. Liver antioxidant enzyme activities 262-5. BAX & Bcl-2 29Ⅳ. Discussion 34Ⅴ. Conclusion 43References 44Abstract 56
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