지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 발행연도
- 2013
- 저작권
- 동의대학교 논문은 저작권에 의해 보호받습니다.
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Objective : This study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice.
Methods : Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE+MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT-PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining.
Results : The promotion of hair growth was observed in GLE+MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE+MTS and MTS compared to CON. The mRNA expression of TGF-α, TGF-β1, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE+MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE+MTS and MTS compared to CON. In the above results, GLE+MTS were more effective than MTS.
Conclusions : These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.
Key words : Ganoderma lucidum extract, alopecia, C57BL/6N, microneedle therapy system
Methods : Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE+MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT-PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining.
Results : The promotion of hair growth was observed in GLE+MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE+MTS and MTS compared to CON. The mRNA expression of TGF-α, TGF-β1, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE+MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE+MTS and MTS compared to CON. In the above results, GLE+MTS were more effective than MTS.
Conclusions : These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.
Key words : Ganoderma lucidum extract, alopecia, C57BL/6N, microneedle therapy system
목차
Ⅰ. 서 론 1Ⅱ. 재료 및 방법 31. 시약 32. 실험동물 33. 영지추출물 조제 44. 미세다륜침 45. 실험방법 46. 피부의 육안적 관찰 및 털 길이 측정 57. 체중 및 장기 무게 측정 58. RNA extraction and RT-PCR 59. Western blotting 610. DNA 및 단백질 밴드의 정량 711. 피부조직의 조직학적 관찰 712. 통계처리 7Ⅲ. 결 과 81. 발모현상 관찰 82. 모발의 밀도, 굵기 및 길이 변화에 미치는 영향 131) 모발의 밀도 변화 132) 모발의 굵기 변화 153) 모발의 길이 변화 173. 체중변화에 미치는 영향 184. 장기무게 변화에 미치는 영향 195. 모낭 및 모발의 성장조절 유전자 발현에 미치는 영향 211) TGF-α, TGF-β1 과 IGF-1 유전자 발현 변화 212) PRL과 PL유전자 발현 변화 236. VEGF와 IGF-1 단백질 발현에 미치는 영향 257. 피부조직의 광학현미경적 관찰소견 27Ⅳ. 고 찰 28Ⅴ. 결 론 34참고문헌 35Abstract 38
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