6. 인삼 질적 형질특성에 따라 6 그룹으로 분류되었으며, Ⅰ 그룹은 1.7%, Ⅱ 그룹은 1.7%, Ⅲ 그룹은 11.7%, Ⅳ 그룹은 1.7%, Ⅴ 그룹은 80%, Ⅵ 그룹은 3.3%가 속하였다.
7. RAPD primer를 사용하여 PCR을 수행한 결과에서 최종적으로 30개를 선발하였다. 최종 선발된 프라이머에서 증폭된 총 band수는 166개이고, 각 primer에 의해 증폭된 DNA 단편의 수는 최소 2개에서 최대 8개로 다양하였으며, primer 한 개당 평균 5.5개의 DNA 단편이 증폭되었다.
8. 유사도계수 0.69을 기준으로 종국과 한국유전자원 60개의 군집분석을 6 그룹으로 분류되었다. Ⅰ 그룹은 6.7%, Ⅱ 그룹은 3.3%, Ⅲ 그룹은 40%, Ⅳ 그룹은 23.3%, Ⅴ 그룹은 13.3%, Ⅵ 그룹은 13.3%가 속하였다.
These studies were conducted to obtain the basic information of phenotypical and genotypical characteristics on germplasm of Panax ginseng C. A. Meyer collected from China and Korea and to indentify the variations that can be utilized in ginseng breeding programs. The qualitative and quantitative characters were investigated and RAPDs was used to clarify the genetic polymorphisms among germplasm of Panax ginseng C. A. Meyer collected from Korea and China. The genetic characteristics and classifications were compared and analyzed among each characters. The research results are as follows;
1. Stem length were followed the normal frequency distribution ranged from 3.53cm to 40.5cm . About 8% of the germplasm was less than 10cm in stem length, 25% with 10∼20cm, 40% with 20∼30cm, 23% with 30∼40cm and 3% with 40∼50cm. The stem diameter of the germplasm was ranged from 2mm to 11mm. 18% was in 2∼4mm, 32% 4∼6cm, 45% in 6∼8mm and 5% in more than 8mm. Stem number per plant was ranged from 1 to 3. About 50% have a single stem, 45% two stems, and 5% three stems.
2. A non-equal frequency distribution was observed in the petiole number. Majority of the population (60%) produced 3-5 petioles, while only 2% produced less than 3 petioles. The petiole angle of the germplasm was ranged from 28° to 89°; 12% were less than 45°, 38% with 45°∼60°, 28% with 60°∼75° and 22% with more than 75°. Petiole length were followed the normal frequency distribution. About 3% of the germplasm was less than 6cm in Petiole length, 10% with 6∼12cm, 35% with 12∼18cm, 40% with 18∼24cm and 12% with 24∼30cm.
3. The germplasms of Panax ginseng C. A. Meyer Collected from China and Korea were classified to 6 groups; Ⅰ, Ⅱ, Ⅲ, Ⅳ, ⅤandⅥ showing 6.7%, 20.0%, 31.7%, 8.3%, 6.7%, and 26.7%, respectively.
4. Frequency distribution of stem color was 12% of green, 15% of light purple, 36% of purple, and 37% of dark purple. Frequency distribution of petiole color was 17% of green, 8% of light purple, 42% of purple, and 33% of dark purple. Frequency distribution of petiolule color was 12% of green, 5% of light purple, 41% of purple and 42% of dark purple.
5. Frequency distribution of root color was 41% of white, 52% of cream and 7% of yellow. Frequency distribution of degree of seed coat plate was nothing have absence seed coat plaet, 90% have rather seed coat plaet and 10% have many seed coat plaet. Frequency distribution of fruit color was 12% of yellow, 5% of jacinth and 83% of red.
6. The germplasms of Panax ginseng C. A. Meyer Collected from China and Korea were classified to 6 groups; Ⅰ, Ⅱ, Ⅲ, Ⅳ, ⅤandⅥ showing 1.7%, 1.7%, 11.7%, 1.7%, 80% and 3.3%, respectively.
7. A total of 30 primers were selected as the appropriate primers for identification of Germplasm of Panax ginseng C. A. Meyer collected from Korea and China. 30 RAPD loci generated a total of 166 bands. The number of amplified bands for each RAPD primers ranged from 2 to 8 with a mean of 5.5 bands.
8. The germplasms of Panax ginseng C. A. Meyer Collected from China and Korea were classified to 6 groups; Ⅰ, Ⅱ, Ⅲ, Ⅳ, ⅤandⅥ showing 6.7%, 3.3%, 40%, 23.3%, 13.3% and 13.3%, respectively.
목차
I. 서 언 1II. 연 구 사 3III. 재료 및 방법 101. 공시재료 102. 재배방법 103. 양?질적 형질특성 조사 134. RAPD 마커를 이용한 분석 155. 통계처리를 통한 유연관계 분석 17IV. 결과 및 고찰 181. 양적 형질 특성 182. 양적 형질을 이용한 유연관계 293. 질적 형질특성 314. 질적 형질을 이용한 유연관계 375. RAPD 마커를 이용한 유전적 특성 396. RAPD을 이용한 유연관계 457. 양적, 질적형질을 이용한 유연관계 478. 양적?질적 형질과 RAPD 마커를 이용한 유연관계 49V. 적 요 52VI. 인 용 문 헌 54