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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김광엽
- 발행연도
- 2013
- 저작권
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Cholera is a representative water-borne disease, caused by Vibrio cholerae ctx (+). Vibrio cholera El Tor was a main pathogen after 7th pandemic outbreak but, in recent, Vibrio cholera El Tor variant was replaced which had classical phenotype and genotype. In this study, we investigated genotype and antimicrobial resistance pattern of imported Vibrio cholerae El Tor in Korea.
Forty-nine strains used in this study were isolated from 2004 to 2011. PCR methods used for the determination of biotype with ctxB and rstR genes. Antimicrobial susceptibility test was performed with sensititre system and mechanisms of antimicrobials resistance was confirmed with PCR method and sequence analysis. Genetic relation was analyzed with PFGE.
Genetic type showed different results: ctxB gene had only classical type but rstR gene had a classical type, El Tor type and combined type. Twenty strains had antimicrobial resistance against streptomycin, sulfamethoxazole/trimethoprim, nalidixic acid, ciprofloxacin. and two mutations detected in the QRDRs of GyrA (Ser83-Ile) and
ParC (Ser85-Leu). Class1 integron were present in all resistant strains . PFGE pattern was grouped as cluster B, which was previously determined and showed high relationship with nations and resistance pattern.
Imported Vibrio cholera El Tor was determined all variant which had a classical ctxB gene and consist of CTX prophage. SXT element plays an important role to resistant antimicrobials and PFGE pattern showed high relationship with antimicrobial resistant strains.
Forty-nine strains used in this study were isolated from 2004 to 2011. PCR methods used for the determination of biotype with ctxB and rstR genes. Antimicrobial susceptibility test was performed with sensititre system and mechanisms of antimicrobials resistance was confirmed with PCR method and sequence analysis. Genetic relation was analyzed with PFGE.
Genetic type showed different results: ctxB gene had only classical type but rstR gene had a classical type, El Tor type and combined type. Twenty strains had antimicrobial resistance against streptomycin, sulfamethoxazole/trimethoprim, nalidixic acid, ciprofloxacin. and two mutations detected in the QRDRs of GyrA (Ser83-Ile) and
ParC (Ser85-Leu). Class1 integron were present in all resistant strains . PFGE pattern was grouped as cluster B, which was previously determined and showed high relationship with nations and resistance pattern.
Imported Vibrio cholera El Tor was determined all variant which had a classical ctxB gene and consist of CTX prophage. SXT element plays an important role to resistant antimicrobials and PFGE pattern showed high relationship with antimicrobial resistant strains.
목차
제1장 : 문헌 고찰 1Ⅰ. 서 론 2Ⅱ. 본 론 51. 비브리오균의 특성 5(1) 장염 비브리오균 (Vibrio parahaemolyticus) 6(2) 비브리오 패혈증균 (Vibrio vulnificus) 8(3) 콜레라균 (Vibrio cholerae) 92. 주요 병원성 비브리오균에 의한 식중독 발생 현황 11(1) Vibrio parahaemolyticus에 의한 식중독 발생 현황 11(2) Vibrio vulnificus에 의한 식중독 발생 현황 14(3) Vibrio cholerae에 의한 식중독 발생 현황 15(4) 비브리오균에 의한 국외 식중독 발생 현황 174. 식품에서의 비브리오균 분리 방법 19(1) 비브리오균 분리 방법 195. 국내에서의 식품 매개 비브리오 식중독 예방 활동 20Ⅲ. 결론 및 고찰 22참고문헌 23제2장 : 국내 유입 Vibrio cholerae O1 biotype El Tor의 특성 및 연관성 분석 28Ⅰ. 서 론 29Ⅱ. 재료 및 방법 341. 대상 균주 34(1) 균주 선별 34(2) 균주 동정 및 생물형 확인 342. 유전자 PCR 393. 항균제 내성 및 내성 기전 확인 42(1) Quinolone계 항균제 내성 기전 42(2) SXT element 존재여부 확인 43(3) Integron 존재 유무 확인 434. Pulse-field gelelectrophoresis (PFGE) 45Ⅲ. 결 과 471. Biotype 결정 472. 유전자형 확인 473. 항균제 내성 양상 및 내성 기전 확인 50(1) Quinolone계 항균제 내성 기전 54(2) Streptomycin 항균제 내성 기전 54(3) 항균제 내성 전파기전 544. PFGE를 통한 역학적 관계 57Ⅳ. 결론 및 고찰 59참고문헌 63
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