Coenzyme A is an ubiquitous essential cofactor that plays a central role in the metabolism of carboxylic acids, includng short and long chain fatty acids. CoA is assembled in five steps from pantothenic acid and pathway intermediates are common to both prokaryotes and eukaryotes. Numerous studies have identified the potential of CoA biosynthetic enzymes because CoA compounds, including acetyl-CoA, propionyl-CoA, malonyl-CoA, and methylmalonyl-CoA are essential precursors required for the biosynthesis of polyketides and other complex biomolecules. Dephosphocoenzyme A catalyzes the last step in the biosynthesis of the essential and ubiquitous cofactor CoA in all organisms and pantothenate kinase catalyzes the first step in the biosynthesis of the CoA. From the genome sequence analysis of S.peucetius ATCC 27952, we identified four CoA biosynthetic genes among which dephosphocoenzyme A kianse was targeted as a final step characterization of its catalytic activity for phosphorylation. Here, we report the identification, cloning and characterization of dephosphocoenzyme A kinase form S. peucetius same kinds of pantothenate kinase. The gene encoded a protein of 209 amino acid with a calculated molecular size of 28.6 kDa. The CoaE was expressed in E.coli BL21(DE3) with His-tag fusion protein and purified by immobilized metal affinity chromatography. The enzyme assay of CoaE was carried out based on coupling assay. The gradual decrease in NADH concentration with time clearly indicated the phosphorylating activity of CoaE. Furthermore, S. peucetius are well known for the production of secondary metabolites, doxorubicin(DXR), including anthracyclines with clinical and economical importance. Therefore to study the effect of CoaE-primary metabolism in secondary metabolites production including panK. So, we constructed the expression recombinant plasmid of coaE, coaA, coaAE under strong ermE* promoter to overexpress in S.peucetius to increase CoA poll thereby increasing the intermediate (malonyl-CoA, propionyl-CoA etc.) for antibiotics production. As a result, DXR production was enhanced by 1.5 fold, 2.1 fold by in vivo overexpression of CoaE, CoaAE.