The Effect of Phaseoli Semen Herbal-acupuncture at KI10 in Lipopolysaccharide induced Acute Nephritis in Rats

Kwak Kyu-in
Dept. of Oriental Medicine
Graduate School, DaeJeon University
(Directed by Prof. Lee Hyun, O.M.D., Ph.D.)

Abstract

Objectives :
This study was designed to evaluate the effects of Phaseoli Semen Herbal-acupuncture(PS-HA) at KI10 in acute nephritis induced by lipopolysaccharide(LPS) in rat.

Methods :
The rats were divided into 5 groups, which were control, LPS, PS-HA, NP and saline group. LPS, PS-HA, NP and saline groups were given LPS to induce acute nephritis and control group did not receive LPS. LPS group did not receive any treatment after the onset of acute nephritis. PS-HA, NP and saline group received PS-HA, normal acupuncture, and saline injection at KI10 three times per week, respectively. To evaluate the effect of PS-HA at KI10, the complete blood count, BUN, creatinine, TNF-α, and CINC-1 in serum were measured. To show its effect on renal function, creatinine, and total protein in urine was measured as well as urine output. The level of myeloperoxidase in renal tissue was quantified and complete histology was done in kidney samples obtained from the rats.

Results :
PS-HA group showed a significant reduction in the proportion of WBC and neutrophil, serum BUN, TNF-a, and CINC-1 compared to LPS group. Furthermore, a significant increase in urine output and a decrease in urinary creatinine level, MPO in renal tissue, and number of neutrophils at glomerulus was observed in PS-HA group compared to LPS group

Conclusion :
PS-HA at KI10 was shown to have a significantly effect on treating LPS induced acute nephrits. Therefore, future study is needed to further evaluate the clinical usefulness of PS-HA at KI10 in treating acute nephritis.

Key words : acute nephritis, Phaseoli Semen, Herbal-acupuncture, KI10, LPS