목적 : 포장내분리현상이 두드러진 梔子의 飮片 규격에 대한 연구를 위해 梔子를 부위별로 全梔子 (Gf), 梔子仁 (Gs), 梔子果皮 (Gp)로 나누어 지표물질정량과 항산화효능비교실험을 수행하였다.
방법 : Gf, Gs, Gp를 80% MeOH에 추출하여 각 시료의 Geniposide를 정량하였고, 항산화효능을 비교하기 위하여 MTT assay, ROS generation, NO production을 수행하고 측정하였다.
결과 : 1. Gf, Gp, Gs에 함유된 Geniposide 정량 결과 Gs에서 가장 높게 정량되었다. 2. MTT assay 결과 Gf, Gp, Gs의 500 μg/ml 단독처리군에서 세포독성은 확인할 수 없었다. 3. Gf 500 μg/ml 전처리군에서는 LPS 단독처리군에 비해서 확연한 ROS generation 감소가 나타났다. 그러나 Gp 및 Gs 전처리군에서는 오히려 LPS 단독처리군보다 ROS generation 증가를 확인할 수 있었다. 4. Gp 500 μg/ml 전처리군에서는 LPS 단독처리군에 비해서 유의적인 NO 생성 감소가 나타났다. Gf 전처리군과 Gs 전처리군에서는 오히려 LPS 단독처리군보다 NO 생성이 증가되었다.
결론 : 지표물질이 梔子仁 (Gs)에 가장 많이 함유되었음에도 불구하고 ROS generation은 全梔子에서, NO generation은 梔子果皮에서 효능이 다른 시료에 비해 뛰어났다. 이와 같이 梔子는 각부위별로 지표물질함량과 효능이 균일하지 않으므로 포장내분리현상을 방지할 수 있는 全梔子의 형태로 유통되는 것이 바람직할 것으로 사료된다
Objective: To study the cutting method of Gardeniae fructus characterized by notable segregation phenomenon after packaging, Gardeniae fructus was divided into the intact Gardeniae fructus (Gf), seed of Gardeniae fructus (Gs), and the pericarp of Gardeniae fructus (Gp) according to its part, and then geniposide quantification and antioxidant effect comparison were conducted. Methods: The Gf, Gs, and Gp were extracted using 80% MeOH, followed by quantizing geniposide contained in each group. A MTT assay was conducted and ROS generation and NO production were measured for comparing its antioxidant effect. Results: 1. As a result of quantizing geniposide contained in the Gf, Gp, and Gs, respectively, the geniposide content was shown to be the highest in the Gs. 2. MTT assay showed that no cytotoxicity was observed in the groups treated with Gf, Gp, and Gs, respectively, at a dose of 500 μg/ml . 3. The ROS generation was shown to have more significantly decreased in the group pretreated with Gf 500 μg/ml than in the group treated with LPS. Meanwhile, it was shown to have more increased in the groups pretreated with Gp and Gs, respectively, than in the group treated with LPS. 4. The NO level was shown to have more significantly decreased in the group pretreated with Gp 500 μg/ml than in the group treated with LPS. Meanwhile, it was shown to have more significantly increased in the groups pretreated with Gf and Gs, respectively, than in the group treated with LPS. Conclusion: Although the geniposide content was the highest in the seed of Gardeniae fructus (Gs), the ROS generation level was the highest in the intact Gardeniae fructus (Gf), and the NO generation level was the highest in the pericarp of Gardeniae fructus (Gp). As the geniposide content and antioxidant effect of Gardeniae fructus vary depending on its part, it is recommended that Gardeniae fructus should be distributed as an intact form to prevent segregation after packaging.