지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2025.2
- 수록면
- 44 - 50 (7page)
- DOI
- 10.7783/KJMCS.2025.33.1.44
이용수
초록· 키워드
오류제보하기
Background: In Korea, the medicinal herb Cnidii Rhizoma is manufactured using Cnidium officinale Makino and Ligusticum chuanxiong Hort., with the former being the primary ingredient. C. officinale and L. chuanxiong have similar external appearances, and because their roots are dried for use as medicinal materials, it is difficult to distinguish between them. Therefore, there is a need to develop easy-to-use molecular markers to facilitate the classification of Cnidii Rhizoma varieties and the identification of medicinal materials.
Methods and Results: DNA was extracted from C. officinale and L. chuanxiong and analyzed using an Illumina HiSeq 2500 platform. After de novo assembly, the loci showing polymorphisms between C. officinale and L. chuanxiong were identified. Primers were designed for polymorphic loci, and genotyping was performed. Finally, three nuclear genome-based insertion and deletion (InDel) markers, CoInDel-01, CoInDel-02, and CoInDel-03, were developed. These markers produced a single band at 230, 369, and 245 bp, respectively, in C. officinale, whereas two bands were observed in L. chuanxiong at 230 and 264 bp, 304 and 369 bp, and 197 and 245 bp, respectively.
Conclusion: The three InDel markers developed in this study will be useful for differentiating between C. officinale and L. chuanxiong
Methods and Results: DNA was extracted from C. officinale and L. chuanxiong and analyzed using an Illumina HiSeq 2500 platform. After de novo assembly, the loci showing polymorphisms between C. officinale and L. chuanxiong were identified. Primers were designed for polymorphic loci, and genotyping was performed. Finally, three nuclear genome-based insertion and deletion (InDel) markers, CoInDel-01, CoInDel-02, and CoInDel-03, were developed. These markers produced a single band at 230, 369, and 245 bp, respectively, in C. officinale, whereas two bands were observed in L. chuanxiong at 230 and 264 bp, 304 and 369 bp, and 197 and 245 bp, respectively.
Conclusion: The three InDel markers developed in this study will be useful for differentiating between C. officinale and L. chuanxiong
목차
ABSTRACT
INTRODUCTION
Materials and Methods
RESULTS
DISCUSSION
REFERENCES
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