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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.9
- 수록면
- 109 - 114 (6page)
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Efficient NADH regeneration from its oxidized form (NAD+) is required to support enzyme-based organic synthesis and the pharmaceutical industry. Prominent industrial NADH regeneration methods have shown promise; however, require the continuous addition of substrate, requiring downstream separation and purification. In this study, the utilization of diaphorase which can directly reduce the NAD+to NADH, with strong and stable mediator is presented which is capable of producing bioactive 1,4-NADH, faradaic efficiencies ~83% at varying temperature (20 to 60 °C). The efficiency of this approach as well as its high operational stability (87% of the maximum activity after six days) make it promising strategy for NADH regeneration.
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