지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.9
- 수록면
- 253 - 264 (12page)
- DOI
- 10.5010/JPB.2024.51.024.253
이용수
초록· 키워드
오류제보하기
The Botrytis genus comprises necrotrophic pathogens that damage various horticultural crops world wide. Nicotiana tabacum is used as a model plant because it can produce the next generation within three months and generate millions of seeds from one plant. In the case of crops that have features difficult to transform, N. tabacum can be used for transformation to validate unknown genes.
The objective of this study was to establish inoculation systems of N. tabacum cv Havana SR1 against two pathogens of the Botrytis genus. As a result, it was appro priate to inoculate 10 µL of conidia suspension (2.25 × 105 cells/µL) using unwounded, in vitro cultured leaves, followed by cultivation at 24°C. In addition, to confirm the resistance response to pathogens at the genetic level, the expression of lipoxygenase (LOX) and allene oxide synthase (AOS), which are jasmonic acid (JA) biosynthetic genes involved in signaling when infected with necrotrophic pathogens, was analyzed. The results showed that NtLOX and NtAOS genes were responsive Botrytis inoculation, with peak expression levels observed at 4 hours post-inoculation.
It was also confirmed that LOX and AOS genes are closely related to disease resistance in Botrytis genus. The ino culation system established in this study using N. tabacum is expected to contribute to identifying the function of genes related to resistance to B. cinerea or B. squamosa in crops that are difficult to transform
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