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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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In this study, we investigated eco-friendly physical and enzymatic methods for extracting proteins from Chlorella pyrenoidosa via cell wall decomposition. The physical methods used were hot-water extraction (HW), sonication (S), and ultrasonication (US), whereas the enzymatic methods included two cell wall-decomposing enzymes (cellulase and viscozyme L) and three proteolytic enzymes (microbial protease, papain, and bromelain) at a 1% substrate con- centration. The results showed that HW and S were unsuitable for extracting chlorella proteins because of their low protein extraction yields. Conversely, among the physical treatment methods, US showed the highest protein extrac- tion yield of 16.78±0.47%. However, physical methods can cause protein denaturation due to temperature increases with extended treatment time. In enzymatic treatment, only microbial protease proved effective, achieving the high- est protein extraction yield of 36.26±2.32% after 6 h of treatment. The use of mixed enzymes did not significantly improve the yield compared to treatment with microbial protease alone. This study suggests that microbial protease is an effective method for protein extraction from chlorella and highlights its potential for application in the food industry as foundational data.
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