지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.12
- 수록면
- 761 - 773 (13page)
이용수
초록· 키워드
오류제보하기
BACKGROUND/OBJECTIVES: The infiltration of macrophages into adipose tissue mediates chronic inflammation that is associated with insulin resistance in obesity. Although vitamin E is beneficial against insulin resistance, its impact on adipose tissue inflammation has not been elucidated. This study aims to investigate the effects of α-tocopherol and γ-tocopherol, major vitamin E isoforms, on the interaction between macrophages and adipocytes with regard to obesity-induced inflammation and insulin resistance.
MATERIALS/METHODS: Hypertrophied 3T3-L1 adipocytes were cocultured with RAW 264.7 macrophages and treated with α-tocopherol or γ-tocopherol at 12.5, 25, and 50 µM. The inflammatory cytokines (monocyte chemoattractant protein-1, tumor necrosis factor-α, and interleukin-6) and free fatty acid (FFA) release were measured by assay kits, and nuclear factor-kappaB (NF-κB) and c-Jun NH2 terminal kinase (JNK) signals were evaluated by immunoblotting. Glucose uptake was measured with a fluorescent glucose derivative.
RESULTS: Treatment with α-tocopherol and γ-tocopherol restrained the coculture-induced increase in cytokines and FFA release. γ-Tocopherol exhibited greater suppression of inflammatory cytokines at 12.5 and 25 µM ( P < 0.001). Both tocopherols inhibited NF-κB activation by limiting translocation of NF-κB (p65) to the nucleus, with γ-tocopherol showing a stronger effect compared to α-tocopherol. α-Tocopherol inhibited JNK phosphorylation at 50 μM, whereas γ-tocopherol did not. Furthermore, coculture with macrophages impaired glucose uptake in response to insulin, but both tocopherols restored insulin responsiveness ( P < 0.01).
CONCLUSION: α-Tocopherol and γ-tocopherol effectively mitigate inflammation induced by adipocyte-macrophage interaction, thereby ameliorating coculture-induced insulin resistance. These findings suggest the therapeutic potential of tocopherols in managing obesity-related metabolic dysfunction.
MATERIALS/METHODS: Hypertrophied 3T3-L1 adipocytes were cocultured with RAW 264.7 macrophages and treated with α-tocopherol or γ-tocopherol at 12.5, 25, and 50 µM. The inflammatory cytokines (monocyte chemoattractant protein-1, tumor necrosis factor-α, and interleukin-6) and free fatty acid (FFA) release were measured by assay kits, and nuclear factor-kappaB (NF-κB) and c-Jun NH2 terminal kinase (JNK) signals were evaluated by immunoblotting. Glucose uptake was measured with a fluorescent glucose derivative.
RESULTS: Treatment with α-tocopherol and γ-tocopherol restrained the coculture-induced increase in cytokines and FFA release. γ-Tocopherol exhibited greater suppression of inflammatory cytokines at 12.5 and 25 µM ( P < 0.001). Both tocopherols inhibited NF-κB activation by limiting translocation of NF-κB (p65) to the nucleus, with γ-tocopherol showing a stronger effect compared to α-tocopherol. α-Tocopherol inhibited JNK phosphorylation at 50 μM, whereas γ-tocopherol did not. Furthermore, coculture with macrophages impaired glucose uptake in response to insulin, but both tocopherols restored insulin responsiveness ( P < 0.01).
CONCLUSION: α-Tocopherol and γ-tocopherol effectively mitigate inflammation induced by adipocyte-macrophage interaction, thereby ameliorating coculture-induced insulin resistance. These findings suggest the therapeutic potential of tocopherols in managing obesity-related metabolic dysfunction.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
α-Tocopherol and γ-tocopherol decrease inflammatory response and insulin resistance during the interaction of adipocytes and macrophages
Nutrition Research and Practice
2024 .12
-
Vitamin E status of 20- to 59-year-old adults living in the Seoul metropolitan area of South Korea
Nutrition Research and Practice
2015 .04
-
Vitamin E status of 20- to 59-year-old adults living in the Seoul metropolitan area of South Korea
Nutrition Research and Practice
2015 .04
-
한국 성인의 비타민 E 섭취량 및 급원식품군의 현황 및 추이 : 제 1~6기 국민건강 영양조사 자료를 이용하여
Journal of Nutrition and Health
2017 .10
-
Resveratrol suppresses inflammatory responses and improves glucose uptake in adipocytes interacted with macrophages
Genes & Genomics
2016 .01
-
Electrostatic Collection of Viable Airborne Coronavirus and Influenza Virus via an α-Tocopherol-based Antioxidant-sampling Solution
한국대기환경학회 학술대회논문집
2021 .10
-
Retinol, α-tocopherol, and selected minerals in breast milk of lactating women with full-term infants in South Korea
Nutrition Research and Practice
2017 .02
-
Retinol, α-tocopherol, and selected minerals in breast milk of lactating women with full-term infants in South Korea
Nutrition Research and Practice
2017 .02
-
Gamma-tocopherol ameliorates hyperglycemia-induced hepatic inflammation associated with NLRP3 inflammasome in alloxan-induced diabetic mice
Nutrition Research and Practice
2019 .10
-
Genome‑wide association study of vitamin E using genotyping by sequencing in sesame (Sesamum indicum)
Genes & Genomics
2019 .01
-
Similarities and differences between alpha-tocopherol and gamma-tocopherol in amelioration of inflammation, oxidative stress and pre-fibrosis in hyperglycemia induced acute kidney inflammation
Nutrition Research and Practice
2016 .02
-
Similarities and differences between alpha-tocopherol and gamma-tocopherol in amelioration of inflammation, oxidative stress and pre-fibrosis in hyperglycemia induced acute kidney inflammation
Nutrition Research and Practice
2016 .02
-
Pear pomace ethanol extract improves insulin resistance through enhancement of insulin signaling pathway without lipid accumulation
Nutrition Research and Practice
2017 .06
-
Pear pomace ethanol extract improves insulin resistance through enhancement of insulin signaling pathway without lipid accumulation
Nutrition Research and Practice
2017 .06
-
Is Acetylation a Metabolic Rheostat that Regulates Skeletal Muscle Insulin Action?
Molecules and Cells
2015 .04
-
Cross talk eliminated six-color detection of single cell imaging; application to monitoring obesity-induced insulin resistance in adipocyte and HepG2 cell co-culture
한국분석과학회 학술대회
2017 .11
-
Associations of serum levels of vitamins A, C, and E with the risk of cognitive impairment among elderly Koreans
Nutrition Research and Practice
2018 .04
-
Associations of serum levels of vitamins A, C, and E with the risk of cognitive impairment among elderly Koreans
Nutrition Research and Practice
2018 .04
-
Induction of heme oxygenase-1 with dietary quercetin reduces obesity-induced hepatic inflammation through macrophage phenotype switching
Nutrition Research and Practice
2016 .12
-
Induction of heme oxygenase-1 with dietary quercetin reduces obesity-induced hepatic inflammation through macrophage phenotype switching
Nutrition Research and Practice
2016 .12
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-25-02-091203378