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논문 기본 정보

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학술저널
저자정보
Sengupta Abhishek (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Gupta Tushar (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Chakraborty Aman (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Kulshrestha Sudeepti (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Redhu Ritu (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Bhattacharjya Raya (Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Tiwari Archana (Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India) Narad Priyanka (Systems Biology and Data Analytics Research Lab, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh-201301, India)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제52권 제2호
발행연도
2024.6
수록면
141 - 151 (11page)
DOI
10.48022/mbl.2309.09011

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Isochrysis sp. is a sea microalga that has become a species of interest because of the extreme lipid content and rapid growth rate of this organism indicating its potential for efficient biofuel production. Using genome sequencing/genome-scale modeling for the prediction of Isochrysis sp. metabolic utilities there is high scope for the identification of essential pathways for the extraction of byproducts of interest at a higher rate. In our work, we design and present iIsochr964, a genome-scale metabolic model of Isochrysis sp. including 4315 reactions, 934 genes, and 1879 metabolites, which are distributed among fourteen compartments. For model validation, experimental culture, and isolation of Isochrysis sp. were performed and biomass values were used for validation of the genome-scale model. OptFlux was instrumental in uncovering several novel metabolites that influence the organism's metabolism by increasing the flux of interacting metabolites, such as Malonyl-CoA, EPA, Protein and others. iIsochr964 provides a compelling resource of metabolic understanding to revolutionize its industrial applications, thereby fostering sustainable development and allowing estimations and simulations of the organism metabolism under varying physiological, chemical, and genetic conditions. It is also useful in principle to provide a systemic view of Isochrysis sp. metabolism, efficiently guiding research and granting context to omics data.

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