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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.9
- 수록면
- 131 - 136 (6page)
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초록· 키워드
오류제보하기
In this investigation, a novel design for a well-plate structure was created to optimize antigen-antibody reactions. The main objective during the development process was to enhance the internal structure of the well plate and increase the surface area. To improve efficiency, the newly designed well-plate was conical in shape and featured internal protrusions, or fins, which increased the surface area per unit volume by 1.45 times compared to standard plates. The performance of the newly developed well plate was assessed using a sandwich CLEIA system, which demonstrated a detection limit approximately 2.5 times better than that of commercial products. Additionally, the coefficient of variation (CV%) was superior to that of commercial products, with inter-assay CV(%) ≤ 11 and intra-assay CV(%) ≤ 9, compared with inter-assay CV(%) ≤ 15 and intra-assay CV(%) ≤ 10 for commercial products. Furthermore, the newly designed well plate demonstrated higher reaction efficiency, even with smaller sample volumes (25~50 μL) compared to the 50~100 μL typically required by commercial well plates. The incorporation of fine patterns increases the number of active sites available for interaction with the samples, thereby significantly enhancing the reaction sensitivity and overall performance.
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