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논문 기본 정보

자료유형
학술저널
저자정보
Jang Sehyeon (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea) Kim San (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea) Kim Se Jeong (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea) Kim Jun Young (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea) Gu Da Hye (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea) So Bo Ram (COSMAX NBT, INC., Seongnam 13486, Republic of Korea) Ryu Jung A (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research) Park Jeong Min (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research) Yoon Sung Ran (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research) Jung Sung Keun (School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of KoreaResearch Institute of Tailored Food Technology, Kyungpook National University, Daegu 41)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.3
발행연도
2024.3
수록면
644 - 653 (10page)
DOI
10.4014/jmb.2309.09026

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Considering the emergence of various infectious diseases, including the coronavirus disease 2019 (COVID-19), people's attention has shifted towards immune health. Consequently, immuneenhancing functional foods have been increasingly consumed. Hence, developing new immuneenhancing functional food products is needed. Pinus densiflora pollen can be collected from the male red pine tree, which is commonly found in Korea. P. densiflora pollen extract (PDE), obtained by water extraction, contained polyphenols (216.29 ± 0.22 mg GAE/100 g) and flavonoids (35.14 ± 0.04 mg CE/100 g). PDE significantly increased the production of nitric oxide (NO) and reactive oxygen species (ROS) but, did not exhibit cytotoxicity in RAW 264.7 cells. Western blot results indicated that PDE induced the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. PDE also significantly increased the mRNA and protein levels of cytokines and the phosphorylation of IKKα/β and p65, as well as the activation and degradation of IκBα. Additionally, western blot analysis of cytosolic and nuclear fractions and immunofluorescence assay confirmed that the translocation of p65 to the nucleus after PDE treatment. These results confirmed that PDE increases the production of cytokines, NO, and ROS by activating NF-κB. Therefore, PDE is a promising nutraceutical candidate for immune-enhancing functional foods.

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