지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.2
- 수록면
- 187 - 202 (16page)
- DOI
- 10.1007/s13258-023-01479-2
이용수
초록· 키워드
오류제보하기
Background Persicaria maackiana (Regel) is a potential medicinal plant that exerts anti-diabetic effects. However, the lack of genomic information on P. maackiana hinders research at the molecular level.
Objective Herein, we aimed to construct a draft genome assembly and obtain comprehensive genomic information on P. maackiana using high-throughput sequencing tools PacBio Sequel II and Illumina.
Methods Persicaria maackiana samples from three natural populations in Gaecheon, Gichi, and Uiryeong reservoirs in South Korea were used to generate genomic DNA libraries, perform genome de novo assembly, gene ontology analysis, phylogenetic tree analysis, genotyping, and identify microsatellite markers.
Results The assembled P. maackiana genome yielded 32,179 contigs. Assessment of assembly integrity revealed 1503 (93.12%) complete Benchmarking Universal Single-Copy Orthologs. A total of 64,712 protein-coding genes were predicted and annotated successfully in the protein database. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs, 13,778 genes were annotated into 18 categories. Genes that activated AMPK were identified in the KEGG pathway. A total of 316,992 microsatellite loci were identified, and primers targeting the flanking regions were developed for 292,059 microsatellite loci. Of these, 150 primer sets were randomly selected for amplification, and 30 of these primer sets were identified as polymorphic. These primers amplified 3–9 alleles. The mean observed and expected heterozygosity were 0.189 and 0.593, respectively. Polymorphism information content values of the markers were 0.361–0.754.
Conclusion Collectively, our study provides a valuable resource for future comparative genomics, phylogeny, and population studies of P. maackiana.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Isolation and characterization of 20 polynucleotide microsatellite markers in a vulnerable Korean snail, Ellobium chinense, using 454 pyrosequencing
Genes & Genomics
2017 .01
-
Development of 21 polymorphic microsatellite markers for the black-banded sea krait, Laticauda semifasciata (Elapidae: Laticaudinae), and cross-species amplification for two other congeneric species
Genes & Genomics
2018 .01
-
RVFV Universal Primers for Whole Genome Amplification and Sequencing
한국미생물학회 학술대회논문집
2022 .10
-
[PRIMER] 저탄소 주택
BBC 사이언스
2023 .07
-
Genomic insights of Leclercia adecarboxylata strains linked to an outbreak in public hospitals in Mexico
Genes & Genomics
2023 .05
-
tufA gene as molecular marker for freshwater Chlorophyceae
Algae
2016 .01
-
Whole Genome Sequencing of MERS-CoV and Related Virus Using Universal Primers
한국미생물학회 학술대회논문집
2020 .10
-
차세대 염기서열 분석법을 이용한 방어(Seriola quinqueradiata)의 microsatellite 마커의 개발 및 유전적 특성 분석
생명과학회지
2020 .03
-
Primers for Amplification and Deep Sequencing of Alphacoronavirus Genomes
한국미생물학회 학술대회논문집
2017 .04
-
Development and characterization of polymorphic microsatellite markers in northern fulmar, Fulmarus glacialis (Procellariiformes), and cross‑species amplification in eight other seabirds
Genes & Genomics
2019 .01
-
Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains
The Plant Pathology Journal
2018 .04
-
Full-length transcriptome combined with RNA sequence analysis of Fraxinus chinensis
Genes & Genomics
2023 .05
-
Characterization of the global transcriptome and microsatellite marker information for spotted halibut Verasper variegatus
Genes & Genomics
2017 .01
-
[PRIMER] 지구의 핵
BBC 사이언스
2023 .05
-
Development of microsatellite markers and analysis of genetic diversity of Barbatia virescens in the southern coasts of China
Genes & Genomics
2019 .01
-
Development and characterization of 21 microsatellite markers in Daphne kiusiana, an evergreen broad-leaved shrub endemic to Korea and Japan
식물분류학회지
2017 .03
-
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
The Plant Pathology Journal
2015 .09
-
Whole Genome Sequencing of RVFV and Related Virus Using Universal Primers
한국미생물학회 학술대회논문집
2021 .08
-
Development and characterization of simple sequence repeat (SSR) markers based on a full-length c
Genes & Genomics
2017 .01
-
Construction of Synaptic Neural Network for Genetic Interaction Analysis
Journal of The Korean Data Analysis Society
2021 .08
최근 본 자료
댓글(0)
0