지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Plant molecular biofarming has been increasingly studied in recent decades. Many kinds of recombinant immunotherapeutic proteins have been produced in transgenic tobacco plants. However, tobacco mosaic virus (TMV) can damage tobacco plants and cause pathogenic symptoms, which affects plant biomass production. Cigarette sap solution has been used to infect TMV during tobacco cultivation. In this study, we obtained TMV-infected transgenic plants expressing a prostatespecific antigen (PSA) fused to an IgG Fc with KDEL ER retention motif (PSA-IgG FcK). The typical TMV-associated symptoms appeared and increased on plant leaves 5 days after infection. mRNA expression levels and the presence of TMV were confirmed by RT-PCR and transmission electron microscopy analyses. The expression level and purity of the target protein were not significantly different between noninfected and infected transgenic plants. TMV was not found in the purified protein samples from infected plants. Our study showed that TMV pathogen-infected plant biomass can be harvested and processed to obtain therapeutic vaccine proteins.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Literature Cited
참고문헌 (37)
참고문헌 신청
J.M. Alonso / 2013 / The physics of tobacco mosaic virus and virus-based devices in biotechnology / Trends in Biotechnology 31(9):530~538
Fanny Balique / 2012 / Tobacco mosaic virus in cigarettes and saliva of smokers / Journal of Clinical Virology 55(4):374~376
Jennifer Dumont / 2016 / Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives / Critical Reviews in Biotechnology 36(6):1110~1122
Doreen M. Floss / 2009 / Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody / Plant Biotechnology Journal 7(9):899~913
James L. Gulley / 2014 / Immune Impact Induced by PROSTVAC (PSA-TRICOM), a Therapeutic Vaccine for Prostate Cancer / Cancer Immunology Research 2(2):133~141
Fanny Balique / 2012 / Tobacco mosaic virus in cigarettes and saliva of smokers / Journal of Clinical Virology 55(4):374~376
Jennifer Dumont / 2016 / Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives / Critical Reviews in Biotechnology 36(6):1110~1122
Doreen M. Floss / 2009 / Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody / Plant Biotechnology Journal 7(9):899~913
James L. Gulley / 2014 / Immune Impact Induced by PROSTVAC (PSA-TRICOM), a Therapeutic Vaccine for Prostate Cancer / Cancer Immunology Research 2(2):133~141
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