지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Nematode infection in the epithelial tissue of cultured rockfish Sebastes schlegeli was first reported in 2012. Since then, nematode infections have caused serious economic losses in rockfish aquaculture on the west coast of Korea. Taxonomic and life cycle information for this parasite are currently unknown. In this study, 18S rRNA and cytochrome c oxidase subunit I (COI) genes were used for molecular identification and polymerase chain reaction (PCR) to detect the invisible stages of this parasite. Nucleotide sequences of the 18S rRNA of the rockfish nematode showed 98% identity with that of Philometra morii. Therefore, this rockfish nematode was classified to the Philometridae family. However, we could not identify it to genus level using 18S rRNA. Its COI nucleotide sequences shared 85% and 82% identities with those of Bursaphelenchus sinensis and Philometra overstreeti, respectively. In addition, two gene-specific primer sets were designed based on the 18S rRNA gene to detect the intermediate host and nematode larvae. These primers were specific to this rockfish nematode without cross-reacting to other pathogens. The detection limit of the PCR assay using these primers was 1,000 copies of nematoda plasmid DNA. Therefore, the PCR assay described here is suitable for the detection of nematode DNA within rockfish. In addition, this PCR assay could be used to detect nematode larvae and the intermediate host.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
선충 유전체 비교 분석을 통한 식물기생선충의 공통 유전자 발굴 및 특성 분석
한국국제농업개발학회지
2016 .01
-
Recent research on enhanced resistance to parasitic nematodes in sweetpotato
Plant Biotechnology Reports
2019 .01
-
Past, present and future of plant quarantine nematodes in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
DEVELOPMENT OF PCR ASSAY FOR DETECTION OF NEMATODA IN ROCKFISH Sebastes schlegeli
한국수산과학회 양식분과 학술대회
2015 .05
-
A root-lesion nematode problem on strawberry
한국농약과학회 학술발표대회 논문집
2018 .10
-
Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea
Fisheries and Aquatic Sciences
2016 .01
-
Expression of Meloidogyne incognita PolA1 hairpin RNA reduced nematode multiplication in transgenic tomato
Plant Biotechnology Reports
2019 .01
-
Current status and prospects of plant parasitic nematodes in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
한국의 잔디기생선충 연구사
Weed&Turfgrass Science
2024 .06
-
Discrimination between three root-knot nematodes based on morphological and molecular characterizations
한국농약과학회 학술발표대회 논문집
2018 .10
-
Distribution of soybean cyst nematodes in Gyeongsang provinces in 2018
한국농약과학회 학술발표대회 논문집
2018 .10
-
Potentials of Weeds as Bio-control Agents to Plant-Parasitic Nematodes
한국잡초학회 별책(학술대회 초록집)
2022 .03
-
Expression analysis of sweetpotato NADPH oxidase-encoding Rboh genes in response to infection with the root-knot nematode Meloidogyne incognita
Plant Biotechnology Reports
2020 .01
-
Survey of root-knot nematodes on yam grown fields in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
Survey of plant parasitic nematode on soybean fields in Jeonbuk province
한국농약과학회 학술발표대회 논문집
2018 .10
-
Survey of plant-parasitic nematodes on pulse crops in Chungnam province in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
Comparison of nematicidal efficacy to clover cyst nematode, Heterodera trifolii and sugar beet cyst nematode, H. schachtii
한국농약과학회 학술발표대회 논문집
2018 .10
-
How can we verify the effectiveness of nematicides on cyst nematode in Chinese cabbage?
한국농약과학회 학술발표대회 논문집
2019 .10
-
Evaluation of the Nematicidal Potential of Some Botanical Substances against Root-Knot Nematode, Meloidogyne incognita in vitro
한국농약과학회 학술발표대회 논문집
2021 .11
-
Screening of nematicidal active materials against turfgrass parasitic nematodes
한국농약과학회 학술발표대회 논문집
2019 .04
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-151-24-02-090170928