지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.5
- 수록면
- 119 - 131 (13page)
이용수
초록· 키워드
오류제보하기
Importance: The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria in pig farms has become a serious concern and reduced the choice of effective antibiotics.
Objective: This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibiotic-resistant Escherichia coli isolated from pig farms in Banten Province, Indonesia.
Methods: Forty-four antibiotic-resistant E. coli isolates from fecal samples from 44 pig farms in Banten Province, Indonesia, were used as samples. The samples were categorized into 14 clusters. Sequencing was performed using the Oxford Nanopore Technologies MinION platform, with barcoding before sequencing with Nanopore Rapid sequencing gDNA-barcoding (SQK-RBK110.96) according to manufacturing procedures. ARG detection was conducted using ResFinder, and the plasmid replicon was determined using PlasmidFinder.
Results: Three phylogenetic leaves of E. coli were identified in the pig farming cluster in Banten Province. The E. coli isolates exhibited potential resistance to nine classes of antibiotics. Fiftyone ARGs were identified across all isolates, with each cluster carrying a minimum of 10 ARGs. The ant(3"")-Ia and qnrS1 genes were present in all isolates. ARGs in the E. coli pig farming cluster originated mainly from plasmids, accounting for an average of 89.4%.
Conclusions and Relevance: The elevated potential for MDR events, coupled with the dominance of ARGs originating from plasmids, increases the risk of ARG spread among bacterial populations in animals, humans, and the environment.
Objective: This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibiotic-resistant Escherichia coli isolated from pig farms in Banten Province, Indonesia.
Methods: Forty-four antibiotic-resistant E. coli isolates from fecal samples from 44 pig farms in Banten Province, Indonesia, were used as samples. The samples were categorized into 14 clusters. Sequencing was performed using the Oxford Nanopore Technologies MinION platform, with barcoding before sequencing with Nanopore Rapid sequencing gDNA-barcoding (SQK-RBK110.96) according to manufacturing procedures. ARG detection was conducted using ResFinder, and the plasmid replicon was determined using PlasmidFinder.
Results: Three phylogenetic leaves of E. coli were identified in the pig farming cluster in Banten Province. The E. coli isolates exhibited potential resistance to nine classes of antibiotics. Fiftyone ARGs were identified across all isolates, with each cluster carrying a minimum of 10 ARGs. The ant(3"")-Ia and qnrS1 genes were present in all isolates. ARGs in the E. coli pig farming cluster originated mainly from plasmids, accounting for an average of 89.4%.
Conclusions and Relevance: The elevated potential for MDR events, coupled with the dominance of ARGs originating from plasmids, increases the risk of ARG spread among bacterial populations in animals, humans, and the environment.
목차
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청이 논문과 함께 이용한 논문
Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs
Min-Hee Kang
,
Min-Joo Chae
,
Jang-Won Yoon
외 5명
Journal of Veterinary Science
2014
.09
3차원 다공성 구조체의 물리적 자극에 의한 세포 기능 향상 연구
김효재
,
정진무
,
오종현
정보 및 제어 논문집
2021
.04
저병원성 조류인플루엔자 감염에 따른 육계의 면역인자 변화
김덕환
,
김규직
,
노진용
외 4명
한국가금학회지
2020
.12
한국 중서부 지역의 논을 이용하는 조류상
손석준
,
최그린
,
도민석
외 1명
한국조류학회지
2020
.12
성상세포의 포도당 전달과정에 대한 수학적 모델
강혁
,
박선미
,
김대식
한국산업응용수학회 학술대회 논문집
2011
.05
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-24-02-089831410