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논문 기본 정보

자료유형
학술저널
저자정보
양승화 (섬섬바이오) 이여진 (조선대학교) 강희선 (섬섬바이오) 신현재 (조선대학교) 최문희 (섬섬바이오)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.39 No.1(Wn.188)
발행연도
2024.3
수록면
25 - 33 (9page)

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초록· 키워드

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Quercus acuta Thunb (QA) has been reported to contain various polyphenolic and flavonoid components, contributing to its antioxidant, antimicrobial, and antiviral effects. However, most studies have focused on Q. acuta Thunb leaves, and there is limited research comparing the physiological activities of leaves and branches or addressing wrinkle improvement. In this study, we investigated the antioxidant activity and wrinkle inhibition effects of Q. acuta Thunb leaf and branch extracts using hot water, 70% ethanol and 100% ethanol. Additionally, LC-MS/MS analysis was employed to identify the physiologically active components in Q. acuta Thunb leaves and branch extracts. The comparison of antioxidant activities between Q. acuta Thunb leaves and branches revealed higher antioxidant activity in Q. acuta Thunb branch extract, with an IC50 value of 117.54 μg/mL in the ABTS radical scavenging assay. To verify the wrinkle improvement effect, the enzyme inhibition activity of collagenase, elastase, and hyaluronidase was measured. The enzyme inhibition experiment results showed that Q. acuta Thunb branches 100% ethanol extract (QAB3) had enzyme inhibition activity, with IC50 values of 390.40 μg/mL, 309.44 μg/mL and 307.98 μg/mL, respectively. RT-PCR results on epidermal cells (HaCaT) showed a decrease in the expression of MMP-3 and MMP-9, enzymes responsible for collagen degradation. Therefore, QAB3 extract proves to be a highly effective substance for antioxidant activity and wrinkle improvement, suggesting its potential use as a functional material in various beauty products.

목차

Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES

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