In this study, an Internet of Things (IoT) environmental sensor was developed to effectively manage indoor air quality in Negative Pressure Isolation Wards (NPIWs). Using this sensor, environmental performance data of both NPIWs and general patient rooms were analyzed. During the summer, humidity levels were consistently high in all rooms, while in the fall, humidity levels decreased by approximately 30% in Hospital A and 20% in Hospital B. Among the general patient rooms, multi-patient rooms exhibited elevated concentrations of CO₂. PM<SUB>2.5</SUB> concentrations were largely influenced by outdoor air, registering low values in most rooms. However, medical procedures or disinfection processes led to PM<SUB>2.5</SUB> concentrations within hospital rooms spiking to over 50 times the initial levels, taking about 4 hours to recover. The data acquired in this study is expected to serve as foundational information for the effective management of indoor air quality in NPIWs in the future.