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Enhancing generation efficiency of liver organoids in a collagen scaffold using human chemically derived hepatic progenitors
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Type
Academic journal
Author
Myounghoi Kim (Hanyang University College of Medicine) Yohan Kim (Max-Planck-Institute of Molecular Cell Biology and Genetics) Elsy Soraya Salas Silva (Hanyang University College of Medicine) Michael Adisasmita (Hanyang University College of Medicine) Kyeong Sik Kim (Hanyang University College of Medicine)
Journal
The Korean Association of HBP Surgery Annals of Hepato-Biliary-Pancreatic Surgery Vol.27 No.4 KCI Accredited Journals
Published
2023.11
Pages
342 - 349 (8page)

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Enhancing generation efficiency of liver organoids in a collagen scaffold using human chemically derived hepatic progenitors
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Backgrounds/Aims: Liver organoids have emerged as a powerful tool for studying liver biology and disease and for developing new therapies and regenerative medicine approaches. For organoid culture, Matrigel, a type of extracellular matrix, is the most commonly used material. However, Matrigel cannot be used for clinical applications due to the presence of unknown proteins that can cause immune rejection, batch-to-batch variability, and angiogenesis.
Methods: To obtain human primary hepatocytes (hPHs), we performed 2 steps collagenase liver perfusion protocol. We treated three small molecules cocktails (A83-01, CHIR99021, and HGF) for reprogramming the hPHs into human chemically derived hepatic progenitors (hCdHs) and used hCdHs to generate liver organoids.
Results: In this study, we report the generation of liver organoids in a collagen scaffold using hCdHs. In comparison with adult liver (or primary hepatocyte)-derived organoids with collagen scaffold (hALO_C), hCdH-derived organoids in a collagen scaffold (hCdHO_C) showed a 10-fold increase in organoid generation efficiency with higher expression of liver- or liver progenitor-specific markers. Moreover, we demonstrated that hCdHO_C could differentiate into hepatic organoids (hCdHO_C_DM), indicating the potential of these organoids as a platform for drug screening.
Conclusions: Overall, our study highlights the potential of hCdHO_C as a tool for liver research and presents a new approach for generating liver organoids using hCdHs with a collagen scaffold.
#Human primary hepatocytes (hPHs) #Human chemically derived hepatic progenitors (hCdHs) #Collagen organoid

Contents

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
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