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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한임상검사정도관리협회 Journal of Laboratory Medicine And Quality Assurance Laboratory Medicine and Quality Assurance 제45권 제2호
- 발행연도
- 2023.6
- 수록면
- 65 - 69 (5page)
- DOI
- 10.15263/jlmqa.2023.45.2.65
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초록· 키워드
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Background: The purpose of this study was to evaluate the reliability of identification with the strains of Acinetobacter baumannii and non-baumanni Acinetobacter spp, determined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), compared to the molecular genetic method.
Methods: A total of 225 blood isolates of A. baumannii and non-baumanni Acinetobacter spp. were included in this study. Species identification was performed using MALDI-TOF MS, blaOXA-51 polymerase chain reaction (PCR), and RNA polymerase β-subunit (rpoB) PCR-sequencing.
Results: The MALDI-TOF MS results showed 100% category agreement with that of blaOXA-51 PCR, which clearly distinguished A. baumannii from nonbaumanni Acinetobacter spp. The species-concordance rate of non-baumanni Acinetobacter spp. was 73.7% (14/19) on the basis of rpoB sequence results.
Conclusions: MALDI-TOF MS is very useful in distinguishing between A. baumannii and non-baumanni Acinetobacter spp. accurately, which is useful information on the choice of therapeutic agents. However, there was a limitation in correctly identifying non-baumanni Acinetobacter spp.
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