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논문 기본 정보

자료유형
학술저널
저자정보
박규태 (부산대학교) Jae Kyung Lim (Department of Physiology College of Medicine Pusan National University) Eun-Bae Choi (Department of Physiology College of Medicine Pusan National University) Mi-Ju Lim (Department of Physiology College of Medicine Pusan National University) Bo-Young Yun (UVA Surgery Clinic) Dae Kyoung Kim (Pusan National University) Jung Won Yoon (Pusan National University) Yoon Gi Hong (BS The Body Aesthetic Plastic Surgery Clinic) Jae Hoon Chang (UVA Surgery Clinic) Seong Hwan Bae (Department of Plastic and Reconstructive Surgery Pusan National University School of Medicine) Jung Yong Ahn (UVA Surgery Clinic) 김재호 (부산대학교)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제27권
발행연도
2023.3
수록면
1,754 - 1,765 (12page)
DOI
https://doi.org/10.1186/s40824-023-00395-6

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Background Adipose tissue-derived microvascular fragments are functional vessel segments derived from arterioles, capillaries, and veins. Microvascular fragments can be used as vascularization units in regenerative medicine and tissue engineering containing microvascular networks. However, the in vivo therapeutic and vascularization properties of human microvascular fragments have not been investigated. Methods In this study, we isolated microvascular fragments, stromal vascular fractions, and mesenchymal stem cells from human lipoaspirate and studied their therapeutic efficacy and in vivo vasculogenic activity in a murine model of hindlimb ischemia. In addition, in vivo angiogenic activity and engraftment of microvascular fragments into blood vessels were measured using Matrigel plug assay. Results Both microvascular fragments and stromal vascular fractions contain not only mesenchymal stem cells but also endothelial progenitor cells. In a Matrigel plug assay, microvascular fragments increased the number of blood vessels containing red blood cells more than mesenchymal stem cells and stromal vascular fractions did. The engraftment of the microvascular fragments transplanted in blood vessels within the Matrigel plug significantly increased compared to the engraftment of mesenchymal stem cells and stromal vascular fractions. Moreover, intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis compared to that of mesenchymal stem cells or stromal vascular fractions. Furthermore, transplanted microvascular fragments formed new blood vessels in ischemic limbs. Conclusions These results suggest that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo and are highly useful for treating ischemic diseases and in tissue engineering. Graphical Abstract Adipose tissue-derived microvascular fragments are vascularization units in regenerative medicine and tissue engineering containing microvascular networks. Intramuscular injection of microvascular fragments markedly increased blood flow in the ischemic hindlimbs and alleviated tissue necrosis. The present study suggests that microvascular fragments show improved engraftment efficiency and vasculogenic activity in vivo andare highly useful for treating ischemic diseases and in tissue engineering.

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