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논문 기본 정보

자료유형
학술저널
저자정보
Cai Xiaoteng (Beijing University of Agriculture People’s Republic of China) Liu Jialin (Beijing University of Agriculture People’s Republic of China) Zhao Fukuan (Beijing University of Agriculture People’s Republic of China) Wang Xiaoqin (Beijing University of Agriculture People’s Republic of China)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.45 No.5
발행연도
2023.5
수록면
593 - 610 (18page)
DOI
10.1007/s13258-022-01335-9

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Background Hemerocallis fulva is an important ground cover plant widely used in urban greening. The analysis of the molecular mechanism underlying the drought response of H. fulva can lay a foundation for improving its adaptability and expanding its planting area. Objective To reveal the drought response mechanisms of H. fulva, identify candidate unigenes associated with drought response, and lay a foundation for further unigenes functional study and drought resistance improvement of H. fulva via genetic engineering. Methods RNA was isolated from H. fulva under different experimental conditions. De novo transcriptomic analysis of the samples was performed to screen drought response unigenes. The transcriptional changes of candidate drought response unigenes were verified by quantitative real-time PCR. Results The differentially expressed unigenes and their functions were analyzed after H. fulva treated by PEG-simulated drought stress and rewatering. The candidate unigenes, associated with H. fulva drought response, were identified after transcriptome analysis. Then, the transcription level of drought response unigenes of H. fulva under different conditions was further verified. Abscisic acid, protein phosphorylation, sterol biosynthesis and ion transport were involved in drought response with quick restore in H. fulva. The response unigenes, involved in hormone (ABA, JA, CK and GA) signaling pathways, defense response, high light response, karrikin response and leaf shaping, can maintain at changed expression levels even after stress withdraw. Conclusion Hemerocallis fulva has unique drought response mechanism. Negative regulation mechanism may play more important roles in drought response of H. fulva. The analysis of candidate unigenes, associated with drought response, lays a foundation for further drought resistance improvement of H. fulva.

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