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논문 기본 정보

자료유형
학술저널
저자정보
Choi Mi Jeong (Biomedical Biotechnology Research Institute Co. Ltd. Goyang 10326 Republic of Korea) Kim Yu Ri (Biomedical Biotechnology Research Institute Co. Ltd. Goyang 10326 Republic of Korea)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제51권 제1호
발행연도
2023.3
수록면
43 - 52 (10page)
DOI
10.48022/mbl.2210.10007

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The purpose of this study is to confirm the antioxidant, black hair, and hair growth effects of the N. jatamansi, O. basilicum, and C. sativus mixed extracts that pharmacological efficacy has been verified. Accordingly, four samples (NOC-1 to-4) produced under different extraction conditions were prepared and the results are as follows. First, all samples showed an increase in antioxidant content in a concentration-dependent manner from the results of antioxidant efficacy. In particular, NOC-4 extracted by steaming and ultrasonic methods showed the highest antioxidant effect among the four samples. As a result of analysis of the amount of melanin production in mouse melanoma cells, NOC-4 with concentration of 500 µg/ml showed higher melanin production compared to the control group, so the black hair efficacy was the best. Also, in the hair growth test results, it was found that the hair growth was the best at 0.94 ± 0.10 mm at experimental group orally administered with 500 mg/kg of NOC-4. In addition, as a result of cytotoxicity analysis in mouse melanoma cells, the safety of samples was demonstrated by maintaining cell viability of 95% or more at all concentrations. These results suggest that the steaming and ultrasonic extraction method increased the extraction yield of active ingredients for antioxidant, melanin, and hair generation, thereby affecting physiological activity. Based on these results, if the steaming and ultrasonic extraction methods are applied to the mixed extraction of N. jatamansi, O. basilicum, and C. sativus, it is judged that the practical potential as a natural material for black hair and hair growth agents will increase.

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