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논문 기본 정보

자료유형
학술저널
저자정보
Jin-Sung Choi (The Catholic University of Korea) Hye Suk Lee (The Catholic University of Korea)
저널정보
한국질량분석학회 Mass Spectrometry Letters Mass Spectrometry Letters Vol.13 No.4
발행연도
2022.12
수록면
95 - 105 (11page)

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초록· 키워드

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Amatoxin-induced mushroom poisoning starts with nonspecific symptoms of toxicity but hepatic damage may follow, resulting in the rapid development of liver insufficiency and, ultimately, coma and death. Accurate detection of amatoxins, such as α-, β-, and γ-amanitin, within the first few hours after presentation is necessary to improve the therapeutic outcomes of patients. Therefore, analytical methods for the identification and quantification of α-, β-, and γ-amanitin in biological samples are necessary for clinical and forensic toxicology. This study presents a literature review of the analytical techniques available for amatoxin detection in biological matrices, and established an inventory of liquid chromatography (LC) techniques with mass spectrometry (MS), ultraviolet (UV) detection, and electrochemical detection (ECD). LC-MS methods using quadrupole tandem mass spectrometry, time-of-flight mass spectrometry, and orbitrap MS are powerful analytical techniques for the identification and determination of amatoxins in plasma, urine, serum, and tissue samples, with high sensitivity, specificity, and reproducibility compared to LC with UV and ECD, enzyme-linked immunoassay, and capillary electrophoresis methods.

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Abstract
Introduction
Amatoxins
Analytical methods of amatoxins in biological fluids
Sample preparation
LC-MS methods
Conclusions
References

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