지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.8
- 수록면
- 211 - 221 (11page)
- DOI
- 10.7783/KJMCS.2023.31.4.211
이용수
초록· 키워드
오류제보하기
Background: Coffee silver skin is a by-product generated during the processing of coffee, which has recently been cultivated mainly in the southern regions of Korea. Radical scavenging active compounds were analyzed as part of a study of active compounds present in coffee silver skin.
Methods and Results: Hot water extracts and ethanol extracts were prepared at varying concentrations (10% - 95%). Simultaneously with liquid chromatography (LC) separation, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was measured and mass spectrometry (MS) of the identified radical scavenging active compound peak was performed. The analysis results confirmed the presence of three compounds, eicosanoyl-5-hydroxytryptamide, docosanoyl-5-hydroxytryptamide, and tetracosanoyl-5-hydroxytryptamide. In the rapid LC-MS/MS analysis using the multiple reaction monitoring mode, each active compound showed a higher content as the ethanol concentration increased. In particular, the content of each compound was found to be significantly decreased in the extract with an ethanol concentration of 60% or less.
Conclusions: The three identified alkanoyl-5-hydroxytryptamide compounds are reported to possess antioxidant activity and various neurological disease-related effects. Therefore, this analysis method can be applied to research or product development using coffee silver skin.
Methods and Results: Hot water extracts and ethanol extracts were prepared at varying concentrations (10% - 95%). Simultaneously with liquid chromatography (LC) separation, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was measured and mass spectrometry (MS) of the identified radical scavenging active compound peak was performed. The analysis results confirmed the presence of three compounds, eicosanoyl-5-hydroxytryptamide, docosanoyl-5-hydroxytryptamide, and tetracosanoyl-5-hydroxytryptamide. In the rapid LC-MS/MS analysis using the multiple reaction monitoring mode, each active compound showed a higher content as the ethanol concentration increased. In particular, the content of each compound was found to be significantly decreased in the extract with an ethanol concentration of 60% or less.
Conclusions: The three identified alkanoyl-5-hydroxytryptamide compounds are reported to possess antioxidant activity and various neurological disease-related effects. Therefore, this analysis method can be applied to research or product development using coffee silver skin.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
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